Name: Anti-MRPL2 Antibody (CAB14404)
Brand: Cell Biology Antibodies 5
SKU: CAB14404
Availability: OutOfStock

Antibody Name:	Anti-MRPL2 Antibody
Antibody SKU:	CAB14404
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-305 of human MRPL2 (NP_057034.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	Mouse kidney, Mouse lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-305 of human MRPL2 (NP_057034.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MALC ALTR ALRS LNLA PPTV AAPA PSLF PAAQ MMNN GLLQ QPSA LMLL PCRP VLTS VALN ANFV SWKS RTKY TITP VKMR KSGG RDHT GRIR VHGI GGGH KQRY RMID FLRF RPEE TKSG PFEE KVIQ VRYD PCRS ADIA LVAG GSRK RWII ATEN MQAG DTIL NSNH IGRM AVAA REGD AHPL GALP VGTL INNV ESEP GRGA QYIR AAGT CGVL LRKV NGTA IIQL PSKR QMQV LETC VATV GRVS NVDH NKRV IGKA GRNR WLGK RPNS GRWH RKGG WAGR KIRP LPPM KSYV KLPS ASAQ S
Gene ID:	51069
Uniprot:	Q5T653
Cellular Location:	Mitochondrion
Calculated MW:	33kDa
Observed MW:	33kDa

Synonyms:

MRPL2, CGI-22, MRP-L14, RPML14

Background:

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the EcoL2 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 12q. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

UniProt Protein Function:	MRPL2: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the EcoL2 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 12q. [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Protein type:Ribosomal; Mitochondrial; RNA-binding; Motility/polarity/chemotaxis; Translation Chromosomal Location of Human Ortholog: 6p21.3 Cellular Component: mitochondrial inner membrane; mitochondrial large ribosomal subunit Molecular Function:structural constituent of ribosome Biological Process: mitochondrial translation
NCBI Summary:	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the EcoL2 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 12q. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2014]
UniProt Code:	Q5T653
NCBI GenInfo Identifier:	118573672
NCBI Gene ID:	51069
NCBI Accession:	Q5T653.2
UniProt Secondary Accession:	Q5T653,Q8WUL1, Q96Q56, Q9Y311, B2RC56,
UniProt Related Accession:	Q5T653
Molecular Weight:	33,301 Da
NCBI Full Name:	39S ribosomal protein L2, mitochondrial
NCBI Synonym Full Names:	mitochondrial ribosomal protein L2
NCBI Official Symbol:	MRPL2
NCBI Official Synonym Symbols:	CGI-22; RPML14; MRP-L14
NCBI Protein Information:	39S ribosomal protein L2, mitochondrial
UniProt Protein Name:	39S ribosomal protein L2, mitochondrial
Protein Family:	39S ribosomal protein
UniProt Gene Name:	MRPL2
UniProt Entry Name:	RM02_HUMAN

Western blot analysis of extracts of various cell lines, using MRPL2 antibody (CAB14404) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

MRPL2 Antibody (PACO10578)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)