Signal Transduction Antibodies 2

Anti-MRPL18 Antibody (CAB16231)

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SKU:
CAB16231
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-MRPL18 Antibody
Antibody SKU:CAB16231
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-92 of human MRPL18 (NP_054880.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:HepG2, K-562
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-92 of human MRPL18 (NP_054880.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MALR SRFW GLFS VCRN PGCR FAAL STSS EPAA KPEV DPVE NEAV APEF TNRN PRNL ELLS VARK ERGW RTVF PSRE FWHR LRVI RTQH HVEA
Gene ID:29074
Uniprot:Q9H0U6
Cellular Location:
Calculated MW:20kDa
Observed MW:18kDa
Synonyms:MRPL18, HSPC071, L18mt, MRP-L18
Background:This nuclear gene encodes a protein component of the larger 39S subunit of mitochondrial ribosome. This protein may also aid in the import of nuclear-encoded 5S rRNA into mitochondria. Alternative splicing results in multiple transcript variants, most of which are not predicted to encode a protein. A pseudogene of this gene is found on chromosome 16.
UniProt Protein Function:MRPL18: Together with thiosulfate sulfurtransferase (TST), acts as a mitochondrial import factor for the cytosolic 5S rRNA. The precursor form shows RNA chaperone activity; is able to fold the 5S rRNA into an import-competent conformation that is recognized by rhodanese (TST). Both the cytoplasmic and mitochondrial forms are able to bind to the helix IV-loop D in the gamma domain of the 5S rRNA. Belongs to the ribosomal protein L18P family.
UniProt Protein Details:

Protein type:Mitochondrial; Ribosomal; Translation

Chromosomal Location of Human Ortholog: 6q25.3

Cellular Component: extracellular space; mitochondrial inner membrane; mitochondrial ribosome; mitochondrion

Molecular Function:5S rRNA binding; structural constituent of ribosome

Biological Process: mitochondrial translation; organelle organization and biogenesis; translation

NCBI Summary:This nuclear gene encodes a protein component of the larger 39S subunit of mitochondrial ribosome. This protein may also aid in the import of nuclear-encoded 5S rRNA into mitochondria. Alternative splicing results in multiple transcript variants, most of which are not predicted to encode a protein. A pseudogene of this gene is found on chromosome 16. [provided by RefSeq, Jan 2016]
UniProt Code:Q9H0U6
NCBI GenInfo Identifier:41017797
NCBI Gene ID:29074
NCBI Accession:Q9H0U6.1
UniProt Secondary Accession:Q9H0U6,Q5TAP9, Q9NZW8,
UniProt Related Accession:Q9H0U6
Molecular Weight:20,577 Da
NCBI Full Name:39S ribosomal protein L18, mitochondrial
NCBI Synonym Full Names:mitochondrial ribosomal protein L18
NCBI Official Symbol:MRPL18  
NCBI Official Synonym Symbols:L18mt; HSPC071; MRP-L18  
NCBI Protein Information:39S ribosomal protein L18, mitochondrial
UniProt Protein Name:39S ribosomal protein L18, mitochondrial
UniProt Gene Name:MRPL18  
UniProt Entry Name:RM18_HUMAN
Anti-MRPL18 Antibody (CAB16231)Western blot analysis of extracts of various cell lines, using MRPL18 Rabbit pAb (CAB16231) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
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