Name: Anti-MRPL17 Antibody (CAB15603)
Brand: Cell Biology Antibodies 6
SKU: CAB15603
Availability: OutOfStock

Antibody Name:	Anti-MRPL17 Antibody
Antibody SKU:	CAB15603
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 86-175 of human MRPL17 (NP_071344.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	293T, MCF7, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 86-175 of human MRPL17 (NP_071344.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	IPKL FQVL APRY KDQT GGYT RMLQ IPNR SLDR AKMA VIEY KGNC LPPL PLPR RDSH LTLL NQLL QGLR QDLR QSQE ASNH SSHT AQTP GI
Gene ID:	63875
Uniprot:	Q9NRX2
Cellular Location:	Mitochondrion
Calculated MW:	20kDa
Observed MW:	25kDa

Synonyms:

MRPL17, L17mt, LIP2, MRP-L17, MRP-L26, RPL17L, RPML26

Background:

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein.

UniProt Protein Function:	MRPL17: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Protein type:Translation; Mitochondrial; Ribosomal Chromosomal Location of Human Ortholog: 11p15.5-p15.4 Cellular Component: mitochondrial inner membrane; mitochondrial large ribosomal subunit Molecular Function:protein domain specific binding; structural constituent of ribosome Biological Process: mitochondrial genome maintenance; mitochondrial translation; organelle organization and biogenesis
NCBI Summary:	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9NRX2
NCBI GenInfo Identifier:	11596859
NCBI Gene ID:	63875
NCBI Accession:	NP_071344.1
UniProt Secondary Accession:	Q9NRX2,Q6IAH8, Q96Q53, Q9C066, D3DQU3,
UniProt Related Accession:	Q9NRX2
Molecular Weight:	20,050 Da
NCBI Full Name:	39S ribosomal protein L17, mitochondrial
NCBI Synonym Full Names:	mitochondrial ribosomal protein L17
NCBI Official Symbol:	MRPL17
NCBI Official Synonym Symbols:	LIP2; L17mt; RPL17L; RPML26; MRP-L17; MRP-L26
NCBI Protein Information:	39S ribosomal protein L17, mitochondrial; LYST-interacting protein 2; LYST-interacting protein LIP2
UniProt Protein Name:	39S ribosomal protein L17, mitochondrial
UniProt Synonym Protein Names:	LYST-interacting protein 2
Protein Family:	39S ribosomal protein
UniProt Gene Name:	MRPL17
UniProt Entry Name:	RM17_HUMAN

Western blot analysis of extracts of various cell lines, using MRPL17 antibody (CAB15603) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.

Antibodies

MRPL17 Antibody (PACO05004)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)