Description
Antibody Name: | Anti-Mras Antibody (CAB20958) |
Antibody SKU: | CAB20958 |
Antibody Size: | 50µL, 100µL |
Application: | Western blotting |
Reactivity: | Human, Mouse, Rat |
Host Species: | Rabbit |
Immunogen: | Recombinant protein of Human Mras. |
Application: | Western blotting |
Recommended Dilution: | WB 1:500 - 1:2000 |
Reactivity: | Human, Mouse, Rat |
Positive Samples: | HeLa, C6, SH-SY5Y, Mouse lung, Mouse brain, Rat brain, Mouse kidney |
Immunogen: | Recombinant protein of Human Mras. |
Purification Method: | Affinity purification |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
Isotype: | IgG |
Sequence: | Email for sequence |
Cellular Location: | Cell membrane, Cytoplasmic side, Lipid-anchor |
Calculated MW: | 15kDa/23kDa |
Observed MW: | 24KDa |
Synonyms: | MRAS, M-RAs, R-RAS3, RRAS3 |
Background: | This gene encodes a member of the Ras family of small GTPases. These membrane-associated proteins function as signal transducers in multiple processes including cell growth and differentiation, and dysregulation of Ras signaling has been associated with many types of cancer. The encoded protein may play a role in the tumor necrosis factor-alpha and MAP kinase signaling pathways. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
Western blot analysis of extracts of various cell lines, using Mras antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s. |