Name: Anti-MPP5 Antibody (CAB13878)
Brand: Signal Transduction Antibodies 1
SKU: CAB13878
Availability: OutOfStock

Antibody Name:	Anti-MPP5 Antibody
Antibody SKU:	CAB13878
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 376-576 of human MPP5 (NP_071919.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Mouse
Positive Samples:	Mouse heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 376-576 of human MPP5 (NP_071919.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GDIL HVIS QEDP NWWQ AYRE GDED NQPL AGLV PGKS FQQQ REAM KQTI EEDK EPEK SGKL WCAK KNKK KRKK VLYN ANKN DDYD NEEI LTYE EMSL YHQP ANRK RPII LIGP QNCG QNEL RQRL MNKE KDRF ASAV PHTT RSRR DQEV AGRD YHFV SRQA FEAD IAAG KFIE HGEF EKNL YGTS IDSV RQVI NSGK ICLL S
Gene ID:	64398
Uniprot:	Q8N3R9
Cellular Location:	Cell junction, Cell membrane, Endomembrane system, Peripheral membrane protein, tight junction
Calculated MW:	73kDa/77kDa
Observed MW:	77kDa

Synonyms:

MPP5, PALS1, Mpp5

Background:

This gene encodes a member of the p55-like subfamily of the membrane-associated guanylate kinase (MAGUK) gene superfamily. The encoded protein participates in the polarization of differentiating cells, has been shown to regulate myelinating Schwann cells (PMID: 20237282), and is one of the components of the Crumbs complex in the retina. Mice which express lower levels of the orthologous protein have retinal degeneration and impaired vision (PMID: 22114289). Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	MPP5: May play a role in tight junctions biogenesis and in the establishment of cell polarity in epithelial cells. May modulate SC6A1/GAT1-mediated GABA uptake by stabilizing the transporter. Required for localization of EZR to the apical membrane of parietal cells and may play a role in the dynamic remodeling of the apical cytoskeleton. Belongs to the MAGUK family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 14q23.3 Cellular Component: nucleoplasm; protein complex; tight junction; lateral loop; cytoplasm; plasma membrane; endomembrane system Molecular Function:protein domain specific binding; protein binding Biological Process: intercellular junction assembly and maintenance; myelin formation; myelin maintenance in the peripheral nervous system; morphogenesis of an epithelial sheet
NCBI Summary:	This gene encodes a member of the p55-like subfamily of the membrane-associated guanylate kinase (MAGUK) gene superfamily. The encoded protein participates in the polarization of differentiating cells, has been shown to regulate myelinating Schwann cells (PMID: 20237282), and is one of the components of the Crumbs complex in the retina. Mice which express lower levels of the orthologous protein have retinal degeneration and impaired vision (PMID: 22114289). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]
UniProt Code:	Q8N3R9
NCBI GenInfo Identifier:	116242632
NCBI Gene ID:	64398
NCBI Accession:	Q8N3R9.3
UniProt Related Accession:	Q8N3R9
Molecular Weight:	73kDa; 77kDa
NCBI Full Name:	MAGUK p55 subfamily member 5
NCBI Synonym Full Names:	membrane palmitoylated protein 5
NCBI Official Symbol:	MPP5
NCBI Official Synonym Symbols:	PALS1
NCBI Protein Information:	MAGUK p55 subfamily member 5
UniProt Protein Name:	MAGUK p55 subfamily member 5
Protein Family:	MAGUK p55 subfamily
UniProt Gene Name:	MPP5
UniProt Entry Name:	MPP5_HUMAN

Western blot analysis of extracts of mouse kidney, using MPP5 antibody (CAB13878) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Antibodies

MPP5 Antibody (PACO10556)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)