Name: Anti-MPP1 Antibody (CAB6298)
Brand: Cell Biology Antibodies 10
SKU: CAB6298
Availability: OutOfStock

Antibody Name:	Anti-MPP1 Antibody
Antibody SKU:	CAB6298
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human MPP1 (NP_002427.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	293T, Mouse kidney, Mouse thymus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human MPP1 (NP_002427.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MTLK ASEG ESGG SMHT ALSD LYLE HLLQ KRSR PEAV SHPL NTVT EDMY TNGS PAPG SPAQ VKGQ EVRK VRLI QFEK VTEE PMGI TLKL NEKQ SCTV ARIL HGGM IHRQ GSLH VGDE ILEI NGTN VTNH SVDQ LQKA MKET KGMI SLKV IPNQ QSRL PALQ MFMR AQFD YDPK KDNL IPCK EAGL KFAT GDII QIIN KDDS NWWQ GRVE GSSK ESAG LIPS PELQ EWRV ASMA QSAP SEAP SCSP FGKK KKYK DKYL AKHS SIFD QLDV VS
Gene ID:	4354
Uniprot:	Q00013
Cellular Location:	Cell projection, Lipid-anchor, Membrane, stereocilium
Calculated MW:	49kDa/52kDa
Observed MW:	52kDa

Synonyms:

MPP1, AAG12, DXS552E, EMP55, MRG1, PEMP

Background:

This gene encodes the prototype of the membrane-associated guanylate kinase (MAGUK) family proteins. MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intercellular junctions. The encoded protein is an extensively palmitoylated membrane phosphoprotein containing a PDZ domain, a Src homology 3 (SH3) motif, and a guanylate kinase domain. This gene product interacts with various cytoskeletal proteins and cell junctional proteins in different tissue and cell types, and may be involved in the regulation of cell shape, hair cell development, neural patterning of the retina, and apico-basal polarity and tumor suppression pathways in non-erythroid cells. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	Essential regulator of neutrophil polarity. Regulates neutrophil polarization by regulating AKT1 phosphorylation through a mechanism that is independent of PIK3CG activity.
NCBI Summary:	This gene encodes the prototype of the membrane-associated guanylate kinase (MAGUK) family proteins. MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intercellular junctions. The encoded protein is an extensively palmitoylated membrane phosphoprotein containing a PDZ domain, a Src homology 3 (SH3) motif, and a guanylate kinase domain. This gene product interacts with various cytoskeletal proteins and cell junctional proteins in different tissue and cell types, and may be involved in the regulation of cell shape, hair cell development, neural patterning of the retina, and apico-basal polarity and tumor suppression pathways in non-erythroid cells. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
UniProt Code:	Q00013
NCBI GenInfo Identifier:	1346575
NCBI Gene ID:	4354
NCBI Accession:	Q00013.2
UniProt Secondary Accession:	Q00013,Q2TSB6, Q5J7V5, B4DZV5, G3XAI1,
UniProt Related Accession:	Q00013
Molecular Weight:	49,854 Da
NCBI Full Name:	55 kDa erythrocyte membrane protein
NCBI Synonym Full Names:	membrane palmitoylated protein 1
NCBI Official Symbol:	MPP1
NCBI Official Synonym Symbols:	MRG1; PEMP; AAG12; EMP55; DXS552E
NCBI Protein Information:	55 kDa erythrocyte membrane protein
UniProt Protein Name:	55 kDa erythrocyte membrane protein
UniProt Synonym Protein Names:	Membrane protein, palmitoylated 1
Protein Family:	Mitochondrial-processing peptidase
UniProt Gene Name:	MPP1
UniProt Entry Name:	EM55_HUMAN

Western blot analysis of extracts of various cell lines, using MPP1 antibody (CAB6298) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s.

Antibodies

MPP1 Antibody (PACO10555)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)