Antibody Name:	Anti-MPO Antibody
Antibody SKU:	CAB1374
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 50-310 of human MPO (NP_000241.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HL-60

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 50-310 of human MPO (NP_000241.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	APAV LGEV DTSL VLSS MEEA KQLV DKAY KERR ESIK QRLR SGSA SPME LLSY FKQP VAAT RTAV RAAD YLHV ALDL LERK LRSL WRRP FNVT DVLT PAQL NVLS KSSG CAYQ DVGV TCPE QDKY RTIT GMCN NRRS PTLG ASNR AFVR WLPA EYED GFSL PYGW TPGV KRNG FPVA LARA VSNE IVRF PTDQ LTPD QERS LMFM QWGQ LLDH DLDF TPEP AARA SFVT GVNC ETSC VQQP PCFP LKIP PNDP RIKN QADC I
Gene ID:	4353
Uniprot:	P05164
Cellular Location:	Lysosome
Calculated MW:	73kDa/83kDa/87kDa
Observed MW:	60KDa

Synonyms:

MPO

Background:

Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils.

UniProt Protein Function:	MPO: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. Homodimer; disulfide-linked. Each monomer consists of a light and a heavy chain. Belongs to the peroxidase family. XPO subfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 1.11.2.2; Oxidoreductase Chromosomal Location of Human Ortholog: 17q23.1 Cellular Component: extracellular space; azurophil granule; mitochondrion; lysosome; nucleus; secretory granule Molecular Function:heparin binding; peroxidase activity; metal ion binding; heme binding; chromatin binding Biological Process: respiratory burst during defense response; response to food; removal of superoxide radicals; response to mechanical stimulus; hydrogen peroxide catabolic process; defense response; response to yeast; response to lipopolysaccharide; response to oxidative stress; defense response to fungus; negative regulation of apoptosis; aging Disease: Myeloperoxidase Deficiency; Alzheimer Disease
NCBI Summary:	Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of netrophils. [provided by RefSeq, Jul 2008]
UniProt Code:	P05164
NCBI GenInfo Identifier:	129825
NCBI Gene ID:	4353
NCBI Accession:	P05164.1
UniProt Secondary Accession:	P05164,Q14862, Q4PJH5, Q9UCL7, A1L4B8,
UniProt Related Accession:	P05164
Molecular Weight:	87,248 Da
NCBI Full Name:	Myeloperoxidase
NCBI Synonym Full Names:	myeloperoxidase
NCBI Official Symbol:	MPO
NCBI Protein Information:	myeloperoxidase
UniProt Protein Name:	Myeloperoxidase
Protein Family:	Myeloperoxidase
UniProt Gene Name:	MPO
UniProt Entry Name:	PERM_HUMAN

	Western blot analysis of extracts of HL-60 cells, using MPO antibody (CAB1374) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded rat spleen using MPO Rabbit pAb (CAB1374) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spleen using MPO Rabbit pAb (CAB1374) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of Mouse bone marrow cells using MPO antibody (CAB1374) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of Human colon carcinoma cells using MPO antibody (CAB1374) at dilution of 1:100. Blue: DAPI for nuclear staining.