Epigenetics & Nuclear Signaling Antibodies 2
Anti-MPHOSPH6 Antibody (CAB15775)
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- SKU:
- CAB15775
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-MPHOSPH6 Antibody |
| Antibody SKU: | CAB15775 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human MPHOSPH6 (NP_005783.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-251MG, A-549, HepG2, Jurkat, HeLa, Raji, Mouse brain, Mouse lung, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human MPHOSPH6 (NP_005783.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAAE RKTR LSKN LLRM KFMQ RGLD SETK KQLE EEEK KIIS EEHW YLDL PELK EKES FIIE EQSF LLCE DLLY GRMS FRGF NPEV EKLM LQMN AKHK AEEV EDET VELD VSDE EMAR RYET LVGT IGKK FARK RDHA NYEE DENG DITP IKAK KMFL KPQD |
| Gene ID: | 10200 |
| Uniprot: | Q99547 |
| Cellular Location: | Cytoplasm, Nucleus, nucleolus |
| Calculated MW: | 19kDa |
| Observed MW: | 22kDa, 23kDa |
| Synonyms: | MPHOSPH6, MPP, MPP-6, MPP6 |
| Background: |
| UniProt Protein Function: | MPHOSPH6: RNA-binding protein that associates with the RNA exosome complex. Involved in the 3'processing of the 7S pre-RNA to the mature 5.8S rRNA and may play a role in recruiting the RNA exosome complex to pre-rRNA; this function may include C1D. Belongs to the MPP6 family. |
| UniProt Protein Details: | Protein type:Cell cycle regulation; Nucleolus Chromosomal Location of Human Ortholog: 16q23.3 Cellular Component: cytoplasm; exosome (RNase complex); nuclear exosome (RNase complex); nucleolus; nucleoplasm; nucleus Molecular Function:protein binding Biological Process: maturation of 5.8S rRNA; rRNA processing |
| UniProt Code: | Q99547 |
| NCBI GenInfo Identifier: | 296438296 |
| NCBI Gene ID: | 10200 |
| NCBI Accession: | Q99547.2 |
| UniProt Secondary Accession: | Q99547,B2RAF0, |
| UniProt Related Accession: | Q99547 |
| Molecular Weight: | 19,024 Da |
| NCBI Full Name: | M-phase phosphoprotein 6 |
| NCBI Synonym Full Names: | M-phase phosphoprotein 6 |
| NCBI Official Symbol: | MPHOSPH6 |
| NCBI Official Synonym Symbols: | MPP; MPP6; MPP-6 |
| NCBI Protein Information: | M-phase phosphoprotein 6 |
| UniProt Protein Name: | M-phase phosphoprotein 6 |
| Protein Family: | M-phase phosphoprotein |
| UniProt Gene Name: | MPHOSPH6 |
| UniProt Entry Name: | MPH6_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MPHOSPH6 antibody (CAB15775) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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