Product Name:	Anti-Mouse TNFα In Vivo Antibody - Low Endotoxin
Product Code:	IVMB0007
Size:	1mg, 5mg, 25mg, 50mg, 100mg
Clone:	TN3-19.12
Protein:	Tumor Necrosis Factor-Alpha
Product Type:	Monoclonal Antibody
Synonyms:	Tumor Necrosis Factor Ligand Superfamily Member 2 (TNFSF2), Cachectin, Differentiation-Inducing Factor (DIF), Necrosin, Cytotoxin
Isotype:	Armenian Hamster IgG
Reactivity:	Mouse
Immunogen:	Recombinant mouse TNFα
Applications:	IP, N, WB
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC >95% by SDS Page
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Product Code	Product Name	Endotoxin Level
IVMB0007	Anti-Mouse TNFα In Vivo Antibody - Low Endotoxin	Low
IVMB0008	Anti-Mouse TNFα In Vivo Antibody - Ultra Low Endotoxin	Ultra Low

Applications:	IP, N, WB
Recommended Usage:	ELISA Each lot of this antibody is quality control tested by ELISA assay. For use of this antibody as a capture, a concentration range of 2 - 6 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of mouse TNF-α recombinant protein ranging from 500 to 4 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
Reactivity:	Mouse

Host Species:	Armenian Hamster
Specificity:	Armenian Hamster Anti-Mouse TNFa (Clone TN3-19.12 ) recognizes Mouse TNFa. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution:	Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
Immunogen:	Recombinant mouse TNFα
Concentration:	≥ 5.0 mg/ml
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC >95% by SDS Page
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

The tumor necrosis factor (TNF-alpha) is a multifaceted polypeptide cytokine known as a mediator of inflammation and immunity.1 It may mediate some of the significant changes in cellular homeostasis which accompany the invasion of the mammalian host by viruses, bacteria, and parasites.2 TNF-α is an acute phase protein which initiates a cascade of cytokines and increases vascular permeability, thereby recruiting macrophage and neutrophils to a site of infection. TNF-α secreted by the macrophage causes blood clotting which serves to contain the infection. TNF-α has been detected in synovial fluid of patients with rheumatoid arthritis. Clone TN3-19.12 antibody can neutralize the bioactivity of natural or recombinant TNF-α