Product Name:	Anti-Mouse/Rat CD126 (IL-6Rα) In Vivo Antibody - Low Endotoxin
Product Code:	IVMB0181
Size:	1mg
Clone:	D7715A7 (15A7)
Protein:	CD126
Product Type:	Monoclonal Antibody
Synonyms:	IL-6 Receptor α chain, IL-6R
Isotype:	Rat IgG2b κ
Reactivity:	Mouse
Immunogen:	OKT4 hybridoma cells
Applications:	FA, FC, N, WB
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC >95% by SDS Page
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Applications:	FA, FC, N, WB
Recommended Usage:	FC The suggested concentration for this D7715A7 (15A7) antibody for staining cells in flow cytometry is ≤ 1.0 µg per 10⁶ in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for this D7715A7 (15A7) antibody for use in western blotting is 1-10 µg/ml.
Reactivity:	Mouse

Host Species:	Rat
Specificity:	Clone D7715A7 (Clone 15A7) recognizes an epitope on mouse CD126.
Antigen Distribution:	CD126 is expressed on activated T and B cells, monocytes, hepatocytes and plasma cells.
Immunogen:	OKT4 hybridoma cells
Concentration:	≥ 5.0 mg/ml
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC >95% by SDS Page
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

CD126 (IL-6Rα) is an 80 kD type I cytokine receptor α chain that is a member of the Ig superfamily that can form a heterodimer with IL-6Rβ chain to form the high affinity IL-6 receptor. The IL-6/IL-6R complex is a potent pleiotropic cytokine that mediates cell growth and differentiation and has significant involvement in immune response. Inadequate production of IL-6 and IL-6R are thought to play a part in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer.