Product Name:	Anti-Mouse CD86 In Vivo Antibody - Ultra Low Endotoxin
Product Code:	IVMB0157
Size:	1mg, 5mg, 25mg, 50mg, 100mg
Clone:	GL1
Protein:	B7-2
Product Type:	Monoclonal Antibody
Synonyms:	B7-2, B70, Ly-58, CD-86
Isotype:	Rat IgG2a κ
Reactivity:	Mouse
Immunogen:	LPS-activated CBA/Ca mouse splenic B cells
Applications:	B, ELISA, WB
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:	<0.5 EU/mg as determined by the LAL method
Purity:	≥98% monomer by analytical SEC >95% by SDS Page
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Product Code	Product Name	Endotoxin Level
IVMB0156	Anti-Mouse CD86 In Vivo Antibody - Low Endotoxin	Low
IVMB0157	Anti-Mouse CD86 In Vivo Antibody - Ultra Low Endotoxin	Ultra Low

Applications:	B, ELISA, WB
Recommended Usage:	ELISA This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml. WB This antibody can be used to detect Human, Mouse and Rat TIM-1 by Western blot analysis at a concentration of 1.0-2.0 µg/ml when used in conjunction with compatible secondary reagents, under either reducing or non-reducing conditions.
Reactivity:	Mouse

Host Species:	Rat
Specificity:	Clone GL-1 recognizes an epitope on mouse CD86.
Antigen Distribution:	CD86 is expressed on activated B and T cells, macrophages, dendritic cells, and astrocytes.
Immunogen:	LPS-activated CBA/Ca mouse splenic B cells
Concentration:	≥ 5.0 mg/ml
Endotoxin Level:	<0.5 EU/mg as determined by the LAL method
Purity:	≥98% monomer by analytical SEC >95% by SDS Page
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

CD86 is an 80kD Ig superfamily member that is involved in immunoglobulin class-switching and activation of NK cell-mediated cytotoxicity. CD80 is closely related to, and works in tandem with CD86 to prime T- cells. CD86 is expressed earlier in the immune response than CD80. The ligation of CD28 on T cells with CD80 and CD86 on APCs co-stimulates T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD86 can also bind to CTLA-4 to deliver an inhibitory signal to T cells.

Technical Datasheet:	View
Protein:	B7-2
Function:	T cell costimulation, Ig class-switching, NK cell cytotoxicity
Ligand/Receptor:	CD28, CD152 (CTLA-4)
Research Area:	Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers