Product Name:	Anti-Mouse CD32/CD16 In Vivo Antibody - Ultra Low Endotoxin
Product Code:	IVMB0028
Size:	1mg, 5mg, 25mg, 50mg, 100mg
Clone:	2.4G2
Protein:	CD32/CD16
Product Type:	Monoclonal Antibody
Synonyms:	Fcγ R III/II, Ly-17
Isotype:	Rat IgG2b
Reactivity:	Mouse
Immunogen:	Sorted pre-B cells
Applications:	FC, IHC FF, IP, PhenoCycler®, WB
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:	<0.5 EU/mg as determined by the LAL method
Purity:	≥98% monomer by analytical SEC >95% by SDS Page
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Product Code	Product Name	Endotoxin Level
IVMB0027	Anti-Mouse CD32/CD16 In Vivo Antibody - Low Endotoxin	Low
IVMB0028	Anti-Mouse CD32/CD16 In Vivo Antibody - Ultra Low Endotoxin	Ultra Low

Applications:	CODEX®, FC, IHC FF, IP, WB
Recommended Usage:	FC The suggested concentration for this 2.4G2 antibody for staining cells in flow cytometry is ≤ 1.0 µg per 10⁶ in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for this 2.4G2 antibody for use in western blotting is 1-10 µg/ml.
Reactivity:	Mouse

Host Species:	Rat
Specificity:	Clone 2.4G2 recognizes the FcγIII and FcγII receptors.
Antigen Distribution:	These receptors are present on B cells, monocyte/macrophages, NK cells, neutrophils, mast cells and dendritic cells.
Immunogen:	Sorted pre-B cells
Concentration:	≥ 5.0 mg/ml
Endotoxin Level:	<0.5 EU/mg as determined by the LAL method
Purity:	≥98% monomer by analytical SEC >95% by SDS Page
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

CD16 is expressed in two forms: CD16a and CD16b. CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. CD16b (FcγRIIIB) is a 48 kD GPI-anchored protein whose extracellular domain is over 95% homologous to that of CD16a. CD16 regulates both phagocytosis and antibody-dependent cell-mediated cytotoxicity. It has been reported that CD16 is involved in Natural Killer Cell activation and plays a role in signal transduction. The receptors, CD32 (FcγRIII) and CD16 (FcγRII), are 40-60 kD and bind antibody-antigen immune complexes and mediate adaptive immune responses.