Product Name:	Anti-Mouse CD25 In Vivo Antibody - Low Endotoxin
Product Code:	IVMB0148
Size:	1mg, 5mg, 25mg, 50mg, 100mg
Clone:	PC61
Protein:	CD25
Product Type:	Monoclonal Antibody
Synonyms:	IL-2Rα, Ly-43, p55, Tac
Isotype:	Rat IgG1 Lambda
Reactivity:	Mouse
Immunogen:	IL-2-dependent cytolytic mouse T-cell clone B6.1
Applications:	B, Depletion, FA, FC, IHC FF, IP, PhenoCycler®, WB
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC >95% by SDS Page
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Product Code	Product Name	Endotoxin Level
IVMB0148	Anti-Mouse CD25 In Vivo Antibody - Low Endotoxin	Low
IVMB0149	Anti-Mouse CD25 In Vivo Antibody - Ultra Low Endotoxin	Ultra Low

Applications:	B, CODEX®, Depletion, FA, FC, IHC FF, IP, WB
Recommended Usage:	FC The suggested concentration for this PC61 antibody for staining cells in flow cytometry is ≤ 1 µg per 10⁶ in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for this PC61 antibody for use in western blotting is 1-10 µg/ml.
Reactivity:	Mouse

Host Species:	Rat
Specificity:	Clone PC61 recognizes an epitope on mouse CD25.
Antigen Distribution:	CD25 is expressed in activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells.
Immunogen:	IL-2-dependent cytolytic mouse T-cell clone B6.1
Concentration:	≥ 5.0 mg/ml
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC >95% by SDS Page
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

CD25, a 55 kD type I transmembrane glycoprotein, has been shown to play roles in lymphocyte differentiation, activation, and proliferation. Many resting memory T cells constitutively express IL2RA. It functions as the receptor for HTLV-1, resulting in its expression on neoplastic cells in adult T cell lymphoma/leukemia. CD25 (sIL-2R) has been used to track disease progression. Some additional clinical applications include Chagas disease, a disease characterized by a decline of CD25 expression on immune cells, and Multiple sclerosis, in which treatments with mAbs target CD25.

Technical Datasheet:	View
Protein:	CD25
Function:	Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132)
Ligand/Receptor:	IL-2
Research Area:	Immunology