Product Name:	Anti-Mouse CD155 (PVR) In Vivo Antibody - Low Endotoxin
Product Code:	IVMB0146
Size:	1mg, 5mg, 25mg, 50mg, 100mg
Clone:	4.24.1
Protein:	CD155
Product Type:	Monoclonal Antibody
Synonyms:	PVR (poliovirus receptor) homolog, Clone 4.24
Isotype:	Rat IgG2a κ
Reactivity:	Mouse
Immunogen:	EL4 transfected with mouse CD155
Applications:	FC
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC >95% by SDS Page
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Product Code	Product Name	Endotoxin Level
IVMB0146	Anti-Mouse CD155 (PVR) In Vivo Antibody - Low Endotoxin	Low
IVMB0147	Anti-Mouse CD155 (PVR) In Vivo Antibody - Ultra Low Endotoxin	Ultra Low

Applications:	FC
Recommended Usage:	FC The suggested concentration for this 4.24.1 antibody for staining cells in flow cytometry is ≤ 0.25 µg per 10⁶ in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Reactivity:	Mouse

Host Species:	Rat
Specificity:	Clone 4.24.1 recognizes an epitope on mouse CD155.
Antigen Distribution:	CD155 is expressed at cell junctions on the primary vascular endothelial cells and is highly expressed on DP thymocytes.
Immunogen:	EL4 transfected with mouse CD155
Concentration:	≥ 5.0 mg/ml
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC >95% by SDS Page
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

CD155 is a transmembrane glycoprotein member of a subfamily of immunoglobulin-like adhesion receptors (nectins). CD155 is commonly known as Poliovirus Receptor (PVR) because of its involvement as a cellular receptor for poliovirus. The normal function of CD155 is to establish intercellular adherens junctions between epithelial cells. Of its 3 extracellular immunoglobulin-like domains (D1, D2, and D3), the virus only recognizes D1. CD155 interacts with both CD226 and CD96 to induce cytotoxicity of NK cells and CTL. The precise role of CD155 in the immune system remains to be elucidated, though it is suspected to be involved in intestinal humoral immune responses. Furthermore, it is thought that CD155 may be used to positively select MHC-independent T cells in the thymus.

Technical Datasheet:	View
Protein:	CD155
Function:	Apart from its function of adhering junction among contacting epithelial cells, the interaction of DNAM-1 (CD226) with its ligands CD155 and CD112 (nectin 2) induces cytotoxcity of NK cells and CD8+ T cells and cytokine secretion.
Ligand/Receptor:	CD226 (DNAM-1), CD96
Research Area:	Immunology, Innate Immunity