Anti-Mouse CD127 (IL-7Rα) In Vivo Antibody - Low Endotoxin

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SKU:
IVMB0044
Product Type:
In Vivo Monoclonal Antibody
Clone:
A7R34
Protein:
CD127
Synonyms:
IL-7Ralpha, Interleukin-7 receptor alpha, IL-7Ralpha chain
Isotype:
Rat IgG2a kappa
Reactivity:
Mouse
Applications:
B, CyTOF®

Description

Product Name:Anti-Mouse CD127 (IL-7Rα) In Vivo Antibody - Low Endotoxin
Product Code:IVMB0044
Size:1mg
Clone:A7R34
Protein:CD127
Product Type:Monoclonal Antibody
Synonyms:IL-7Rα, Interleukin-7 receptor alpha, IL-7Rα chain
Isotype:Rat IgG2a κ
Reactivity:Mouse
Immunogen:IL-7Ra-IgG1 fusion protein
Applications:B, CyTOF®, FA, FC, IF Staining, IHC FF, IP, WB
Formulation:This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:< 1.0 EU/mg as determined by the LAL method
Purity:≥95% monomer by analytical SEC
>95% by SDS Page
Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Applications:B, CyTOF®, FA, FC, IF Staining, IHC FF, IP, WB
Recommended Usage:FC
The suggested concentration for this A7R34 antibody for staining cells in flow cytometry is ≤ 0.25 µg per 106 in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application.
Reactivity:Mouse
Host Species:Rat
Specificity:Clone A7R34 recognizes an epitope on mouse CD127.
Antigen Distribution:IL-7Rα is expressed on bone marrow lymphoid precursors, pro-B cells, monocytes/macrophages, NK-cells, naïve T cells, memory CD4+ and CD8+ T cells, intestinal colorectal and renal carcinoma cell lines, and keratinocytes.
Immunogen:IL-7Ra-IgG1 fusion protein
Concentration:≥ 5.0 mg/ml
Endotoxin Level:< 1.0 EU/mg as determined by the LAL method
Purity:≥95% monomer by analytical SEC
>95% by SDS Page
Formulation:This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

CD127 (IL7R-α) is a 60-90 kD type I cytokine receptor involved in the regulation of lymphopoiesis. The active receptor is an α/γ chain heterodimer. The common γ chain, which also associates with the IL-2 receptor, serves mainly to activate signal transduction via the IL7R complex. CD127 also interacts with the Thymic Stromal Lymphopoietin Receptor (TSLPR). CD127 determines signaling events via its association with cytoplasmic signaling molecules and has been reported to be a useful marker for identifying memory and effector T cells.

Protein:CD127
Function:T cell and immature B cell proliferation and development
Ligand/Receptor:IL-7
Research Area:Immunology
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