Description
Product Name: | Anti-Mouse CD11a [I21/7] In Vivo Antibody - Ultra Low Endotoxin |
Product Code: | IVMB0140 |
Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
Clone: | I21/7 |
Protein: | CD11a |
Product Type: | Monoclonal Antibody |
Synonyms: | LFA-1α |
Isotype: | Rat IgG2a |
Reactivity: | Mouse |
Immunogen: | Whole T cells Lymphoma BW5147 cells |
Applications: | FC |
Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
Endotoxin Level: | <0.5 EU/mg as determined by the LAL method |
Purity: | ≥98% monomer by analytical SEC >95% by SDS Page |
Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
Applications: | FC |
Recommended Usage: | FC The suggested concentration for clone I21/7 antibody for staining cells in flow cytometry is ≤ 0.5-1.0 µg per 106 in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application. |
Reactivity: | Mouse |
Host Species: | Rat |
Specificity: | Clone I21/7 recognizes an epitope on mouse CD11a. |
Antigen Distribution: | CD11a is present on thymocytes, blood lymphocytes, bone marrow cells and certain lymphoma and macrophage-like cell lines. |
Immunogen: | Whole T cells Lymphoma BW5147 cells |
Concentration: | ≥ 5.0 mg/ml |
Endotoxin Level: | <0.5 EU/mg as determined by the LAL method |
Purity: | ≥98% monomer by analytical SEC >95% by SDS Page |
Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
LFA-1α (CD11a) and CD18 are the Integrin alpha-L and beta-2 chains respectively that combine to form LFA-1, a glycoprotein and a member of the Integrin family. Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3, ICAM4 and for F11R. LFA-1 participates in the immunological synapses between CD8+ T lymphocytes and antigen-presenting cells. The absence of LFA-1α or β may induce LAD. The antigen contributes to natural killer cell cytotoxicity, and is involved in various immune phenomena such as leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. The CD11b/CD18 antigen is a heterodimeric surface glycoprotein on leukocytes and belongs to the β2 integrin family. CD11b functions as a receptor for C3bi complement, clotting factor X, fibrinogen and ICAM-1. CD11c forms an α/β heterodimeric glycoprotein (CD11c/CD18 complex) which belongs to the β2 integrin family. The complex binds fibrinogen and reportedly serves as a receptor for iC3b and ICAM-1. During inflammatory responses, it mediates cell to cell interaction and is important in both monocyte adhesion and chemotaxis.
Technical Datasheet: | View |
Protein: | CD11a |
Function: | Intercellular adhesion |
Ligand/Receptor: | ICAM-1 (CD54), ICAM-2(CD102), ICAM-3(CD50) |
Research Area: | Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Signal Transduction |
Rat IgG2a Isotype Control | |
---|---|
Clone | 1-1 |
Isotype | Rat IgG2a |
Endotoxin Level | Ultra Low Endotoxin |
Meet the team!
Shane Costigan
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Abdul Khadim
Sales Executive