Product Name:	Anti-Mouse CD11a [FD441.8] In Vivo Antibody - Ultra Low Endotoxin
Product Code:	IVMB0041
Size:	1mg, 5mg, 25mg, 50mg, 100mg
Clone:	FD441.8
Protein:	CD11a
Product Type:	Monoclonal Antibody
Synonyms:	LFA-1α
Isotype:	Rat IgG2b
Reactivity:	Mouse
Immunogen:	Cloned CTL B18 cells
Applications:	FC
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:	<0.5 EU/mg as determined by the LAL method
Purity:	≥98% monomer by analytical SEC >95% by SDS Page
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Product Code	Product Name	Endotoxin Level
IVMB0040	Anti-Mouse CD11a [FD441.8] In Vivo Antibody - Low Endotoxin	Low
IVMB0041	Anti-Mouse CD11a [FD441.8] In Vivo Antibody - Ultra Low Endotoxin	Ultra Low

Applications:	FC
Recommended Usage:	FC The suggested concentration for this FD441.8 antibody for staining cells in flow cytometry is ≤ .25 µg per 10⁶ in a 100 µl volume or 100 µl of whole blood. Titration of the reagent is recommended for optimal performance for each application. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Reactivity:	Mouse

Host Species:	Rat
Specificity:	Clone FD441.8 recognizes an epitope on mouse CD11a.
Antigen Distribution:	CD11a is present on thymocytes, blood lymphocytes, bone marrow cells and certain lymphoma and macrophage-like cell lines.
Immunogen:	Cloned CTL B18 cells
Concentration:	≥ 5.0 mg/ml
Endotoxin Level:	<0.5 EU/mg as determined by the LAL method
Purity:	≥98% monomer by analytical SEC >95% by SDS Page
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

LFA-1α (CD11a) and CD18 are the Integrin alpha-L and beta-2 chains respectively that combine to form LFA-1, a glycoprotein and a member of the Integrin family. Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3, ICAM4 and for F11R. LFA-1 participates in the immunological synapses between CD8+ T lymphocytes and antigen-presenting cells. The absence of LFA-1α or β may induce LAD. The antigen contributes to natural killer cell cytotoxicity, and is involved in various immune phenomena such as leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. The CD11b/CD18 antigen is a heterodimeric surface glycoprotein on leukocytes and belongs to the β2 integrin family. CD11b functions as a receptor for C3bi complement, clotting factor X, fibrinogen and ICAM-1. CD11c forms an α/β heterodimeric glycoprotein (CD11c/CD18 complex) which belongs to the β2 integrin family. The complex binds fibrinogen and reportedly serves as a receptor for iC3b and ICAM-1. During inflammatory responses, it mediates cell to cell interaction and is important in both monocyte adhesion and chemotaxis.

Technical Datasheet:	View
Protein:	CD11a
Function:	LFA-1 plays a central role in leukocyte intercellular adhesion.
Ligand/Receptor:	ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50)
Research Area:	Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Signal Transduction