Protein Tags
Anti-Mouse anti V5-Tag Monoclonal Antibody (CABE017)
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- SKU:
- CABE017
- Product Type:
- Antibody
- Host Species:
- Mouse
- Isotype:
- IgG
- Antibody Type:
- Monoclonal Antibody
Description
| Antibody Name: | Anti-Mouse anti V5-Tag Monoclonal Antibody |
| Antibody SKU: | CABE017 |
| Antibody Size: | 100 uL |
| Application: | WB IF IP |
| Reactivity: | |
| Host Species: | Mouse |
| Immunogen: | A synthetic peptide corresponding to V5 tag. |
| Application: | WB IF IP |
| Recommended Dilution: | WB 1:1000 - 1:5000 IF 1:50 - 1:200 IP 1:50 - 1:200 |
| Reactivity: | |
| Positive Samples: | 293T |
| Immunogen: | A synthetic peptide corresponding to V5 tag. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | GKPI PNPL LGLD ST |
| Gene ID: | |
| Uniprot: | |
| Cellular Location: | |
| Calculated MW: | |
| Observed MW: | Refer to Figures |
 | Western blot analysis of recombinant V5 protein using Mouse anti V5-Tag mAb (CABE017) at dilution of 1:2000. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
| Synonyms: | V5, V5 tag, V5-tag |
| Background: | Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification. |
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