Protein Tags
Anti-Mouse anti RFP-Tag Monoclonal Antibody (CABE020)
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- SKU:
- CABE020
- Product Type:
- Antibody
- Host Species:
- Mouse
- Isotype:
- IgG
- Antibody Type:
- Monoclonal Antibody
Description
| Antibody Name: | Anti-Mouse anti RFP-Tag Monoclonal Antibody |
| Antibody SKU: | CABE020 |
| Antibody Size: | 100 uL |
| Application: | WB IHC IP |
| Reactivity: | |
| Host Species: | Mouse |
| Immunogen: | Recombinant protein of RFP-Tag |
| Application: | WB IHC IP |
| Recommended Dilution: | WB 1:2000 - 1:5000 IHC 1:50 - 1:200 IP 1:50 - 1:200 |
| Reactivity: | |
| Positive Samples: |
| Immunogen: | Recombinant protein of RFP-Tag |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MASS ENVI TEFM RFKV RMEG TVNG HEFE IEGE GEGR PYEG HNTV KLKV TKGG PLPF AWDI LSPQ FQYG SKVY VKHP ADIP DYKK LSFP EGFK WERV MNFE DGGV ATVT QDSS LQDG CFIY KVKF IGVN FPSD GPVM QKKT MGWE ASTE RLYP RDGV LKGE THKA LKLK DGGH YLVE FKSI YMAK KPVQ LPGY YYVD AKLD ITSH NEDY TIVE QYER TEGR HHLF L |
| Gene ID: | |
| Uniprot: | |
| Cellular Location: | |
| Calculated MW: | 27kDa |
| Observed MW: | Refer to Figures |
 | Western blot analysis of recombinant RFP protein using RFP-tag antibody (CABE020) at dilution of 1:10000. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
| Synonyms: | RFP, RFP tag, RFP-tag |
| Background: | Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes. Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification. |
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