Protein Tags
Anti-Mouse anti His-Tag Monoclonal Antibody (CABE003)
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- SKU:
- CABE003
- Product Type:
- Antibody
- Host Species:
- Mouse
- Isotype:
- IgG
- Antibody Type:
- Monoclonal Antibody
Description
| Antibody Name: | Anti-Mouse anti His-Tag Monoclonal Antibody |
| Antibody SKU: | CABE003 |
| Antibody Size: | 100 uL |
| Application: | WB IF IP |
| Reactivity: | |
| Host Species: | Mouse |
| Immunogen: | A synthetic peptide corresponding to His tag. |
| Application: | WB IF IP |
| Recommended Dilution: | WB 1:2000 - 1:5000 IF 1:50 - 1:200 IP 1:50 - 1:100 |
| Reactivity: | |
| Positive Samples: | 293T |
| Immunogen: | A synthetic peptide corresponding to His tag. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | HHHH HH |
| Gene ID: | |
| Uniprot: | |
| Cellular Location: | |
| Calculated MW: | |
| Observed MW: | Refer to Figures |
 | Western blot analysis of extracts of normal 293T cells and 293T transfected with His-CEND1 protein, using His-Tag antibody (CABE003) at 1:100000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 120s. |
 | Western blot analysis of extracts of normal 293T cells and 293T transfected with EPCAM protein, using His-Tag antibody (CABE003) at 1:20000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of 293T cells using Mouse anti His-Tag mAb (CABE003) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunoprecipitation of over-expressed His-tagged protein in 293T cells incubated using His-tag antibody (CABE003). A mock served as negative control and over-expressed 293T cell lysate served as positive control. |
| Synonyms: | His, His tag, His-tag |
| Background: | Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected by using His antibodies. |
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