Protein Tags
Anti-Mouse anti DDDDK-Tag Monoclonal Antibody (CABE005)
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- SKU:
- CABE005
- Product Type:
- Antibody
- Host Species:
- Mouse
- Isotype:
- IgG
- Antibody Type:
- Monoclonal Antibody
Description
| Antibody Name: | Anti-Mouse anti DDDDK-Tag Monoclonal Antibody |
| Antibody SKU: | CABE005 |
| Antibody Size: | 100 uL |
| Application: | WB IHC IF IP |
| Reactivity: | |
| Host Species: | Mouse |
| Immunogen: | A synthetic peptide corresponding to DDDDK tag. |
| Application: | WB IHC IF IP |
| Recommended Dilution: | WB 1:2000 - 1:5000 IHC 1:50 - 1:500 IF 1:50 - 1:500 IP 1:50 - 1:100 |
| Reactivity: | |
| Positive Samples: | 293T |
| Immunogen: | A synthetic peptide corresponding to DDDDK tag. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | DYKD DDDK |
| Gene ID: | |
| Uniprot: | |
| Cellular Location: | |
| Calculated MW: | |
| Observed MW: | Refer to Figures |
 | Western blot analysis of over-expressed DDDDK-tagged protein in 293T cells using Mouse anti DDDDK-Tag antibody (CABE005) at different dilution.Each lane was loaded with 2 ug cell lysate. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s. |
 | Immunofluorescence analysis of 293T cells using Mouse anti DDDDK-Tag mAb (CABE005). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of HeLa cells transfected with DDDDK-Tag and untreated HeLa cells use Mouse anti DDDDK-Tag mAb(CABE005) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunoprecipitation of over-expressed DDDDK-tagged protein in 293T cells using DDDDK-tag antibody (CABE005). A mock served as negative control and over-expressed 293T cell lysate served as positive control. |
| Synonyms: | DDDDK, DDDDK tag, DDDDK-tag |
| Background: | FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography.A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis and Western blotting. |
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