Product Name:	Anti-Mouse 4-1BB In Vivo Antibody - Ultra Low Endotoxin
Product Code:	IVMB0046
Size:	1mg, 5mg, 25mg, 50mg, 100mg
Clone:	3H3
Protein:	4-1BB
Product Type:	Monoclonal Antibody
Synonyms:	CD137, CD137L, TNFSF9
Isotype:	Rat IgG2a
Reactivity:	Mouse
Immunogen:	Recombinant Mouse CD137 human Fc fusion protein
Applications:	ELISA, FA, WB
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:	<0.5 EU/mg as determined by the LAL method
Purity:	≥98% monomer by analytical SEC >95% by SDS Page
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Product Code	Product Name	Endotoxin Level
IVMB0045	Anti-Mouse 4-1BB In Vivo Antibody - Low Endotoxin	Low
IVMB0046	Anti-Mouse 4-1BB In Vivo Antibody - Ultra Low Endotoxin	Ultra Low

Applications:	ELISA, FA, WB
Recommended Usage:	WB ELISA
Reactivity:	Mouse

Host Species:	Rat
Specificity:	Clone 3H3 recognizes an epitope on mouse 4-1BB.
Antigen Distribution:	4-1BB is expressed on activated B cells and T cells, macrophages, and dendritic cells.
Immunogen:	Recombinant Mouse CD137 human Fc fusion protein
Concentration:	≥ 5.0 mg/ml
Endotoxin Level:	<0.5 EU/mg as determined by the LAL method
Purity:	≥98% monomer by analytical SEC >95% by SDS Page
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

4-1BB (CD137) is a 39 kD transmembrane protein that is a member of the tumor necrosis factor (TNF) receptor family and is a co-stimulatory molecule that plays a role in T-cell-mediated proliferative response. When binding its ligand, CD137 provides costimulatory signals to both CD4 and CD8 T cells via the activation of NF-\uf06bB, c-Jun and p38 downstream pathways. Crosslinking of CD137 boosts T cell proliferation, IL-2 secretion, survival and cytolytic activity. Furthermore, it can increase immune activity to eliminate tumors in mice. Agonistic anti-CD137 antibodies have been reported to stimulate a more intense immune system attack on cancers.