Cell Biology Antibodies 15

Anti-MonoMethyl-Histone H3-R2 Antibody (CAB19645)

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SKU:
CAB19645
Product Type:
Antibody
Applications:
WB
Applications:
IP
Applications:
ChIP
Reactivity:
Human
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-MonoMethyl-Histone H3-R2 Antibody
Antibody SKU:CAB19645
Antibody Size:20uL, 50uL, 100uL
Application:WB IP ChIP
Reactivity:Human, Rat
Host Species:Rabbit
Immunogen:A synthesized peptide derived from human Histone H3 (mono methyl R2).
Application:WB IP ChIP
Recommended Dilution:WB 1:500 - 1:2000 ChIP 1:20 - 1:100
Reactivity:Human, Rat
Positive Samples:HeLa, C6
Immunogen:A synthesized peptide derived from human Histone H3 (mono methyl R2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:8290/8350
Uniprot:Q16695/P68431
Cellular Location:
Calculated MW:15kDa
Observed MW:17KDa
Western blot - MonoMethyl-Histone H3-R2 Rabbit mAb Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H3-R2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Chromatin Immunoprecipitation - MonoMethyl-Histone H3-R2 Rabbit mAb Chromatin immunoprecipitation analysis of extracts of HeLa cells, using MonoMethyl-Histone H3-R2 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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