Description
Antibody Name: | Anti-MonoMethyl-Histone H3-K18 Antibody (CAB20680) |
Antibody SKU: | CAB20680 |
Antibody Size: | 50µL, 100µL |
Application: | Western blotting, Immunohistochemistry, Immunofluorescence |
Reactivity: | Human, Mouse, Rat |
Host Species: | Rabbit |
Immunogen: | A synthetic methylated peptide corresponding to residues surrounding K18 of human histone H3 |
Application: | Western blotting, Immunohistochemistry, Immunofluorescence |
Recommended Dilution: | DB 1:500 - 1:2000 WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 ChIP 1:50 - 1:200 |
Reactivity: | Human, Mouse, Rat |
Positive Samples: | HeLa acid extract, NIH/3T3 acid extract, C6 acid extract, H3 protein |
Immunogen: | A synthetic methylated peptide corresponding to residues surrounding K18 of human histone H3 |
Purification Method: | Affinity purification |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
Isotype: | IgG |
Sequence: | Email for sequence |
Cellular Location: | Chromosome, Nucleus |
Calculated MW: | 15kDa |
Observed MW: | 17KDa |
Synonyms: | H3.4, H3/g, H3FT, H3t, HIST3H3, Histone H3, HIST1H3A |
Background: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. |
Immunofluorescence analysis of U2OS cells using MonoMethyl-Histone H3-K18 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. | |
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using MonoMethyl-Histone H3-K18 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input. | |
Immunofluorescence analysis of NIH/3T3 cells using MonoMethyl-Histone H3-K18 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. | |
Immunohistochemistry of paraffin-embedded rat brain using MonoMethyl-Histone H3-K18 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. | |
Immunohistochemistry of paraffin-embedded mouse lung using MonoMethyl-Histone H3-K18 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. | |
Immunohistochemistry of paraffin-embedded human breast cancer using MonoMethyl-Histone H3-K18 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. | |
Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H3-K18 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s. |