Anti-MonoMethyl-Histone H3-K18 Antibody (CAB20680)

(No reviews yet) Write a Review
SKU:
CAB20680

Description

Antibody Name:Anti-MonoMethyl-Histone H3-K18 Antibody (CAB20680)
Antibody SKU:CAB20680
Antibody Size:50µL, 100µL
Application:Western blotting, Immunohistochemistry, Immunofluorescence
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic methylated peptide corresponding to residues surrounding K18 of human histone H3
Application:Western blotting, Immunohistochemistry, Immunofluorescence
Recommended Dilution:DB 1:500 - 1:2000
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
ChIP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa acid extract, NIH/3T3 acid extract, C6 acid extract, H3 protein
Immunogen:A synthetic methylated peptide corresponding to residues surrounding K18 of human histone H3
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Cellular Location:Chromosome, Nucleus
Calculated MW:15kDa
Observed MW:17KDa
Synonyms:H3.4, H3/g, H3FT, H3t, HIST3H3, Histone H3, HIST1H3A
Background:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Immunofluorescence analysis of U2OS cells using MonoMethyl-Histone H3-K18 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunofluorescence analysis of U2OS cells using MonoMethyl-Histone H3-K18 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using MonoMethyl-Histone H3-K18 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.Chromatin immunoprecipitation analysis of extracts of HeLa cells, using MonoMethyl-Histone H3-K18 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Immunofluorescence analysis of NIH/3T3 cells using MonoMethyl-Histone H3-K18 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunofluorescence analysis of NIH/3T3 cells using MonoMethyl-Histone H3-K18 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded rat brain using MonoMethyl-Histone H3-K18 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.Immunohistochemistry of paraffin-embedded rat brain using MonoMethyl-Histone H3-K18 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse lung using MonoMethyl-Histone H3-K18 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.Immunohistochemistry of paraffin-embedded mouse lung using MonoMethyl-Histone H3-K18 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human breast cancer using MonoMethyl-Histone H3-K18 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.Immunohistochemistry of paraffin-embedded human breast cancer using MonoMethyl-Histone H3-K18 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H3-K18 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H3-K18 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
View AllClose

Additional Information

Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
View AllClose

Related Products