Name: Anti-MOCS3 Antibody (CAB7367)
Brand: Metabolism Antibodies 2
SKU: CAB7367
Availability: OutOfStock

Antibody Name:	Anti-MOCS3 Antibody
Antibody SKU:	CAB7367
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 271-460 of human MOCS3 (NP_055299.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	HepG2, Jurkat, Mouse ovary, Mouse heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 271-460 of human MOCS3 (NP_055299.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	YSGS LLLF DALR GHFR SIRL RSRR LDCA ACGE RPTV TDLL DYEA FCGS SATD KCRS LQLL SPEE RVSV TDYK RLLD SGAF HLLL DVRP QVEV DICR LPHA LHIP LKHL ERRD AESL KLLK EAIW EEKQ GTQE GAAV PIYV ICKL GNDS QKAV KILQ SLSA AQEL DPLT VRDV VGGL MAWA AKID GTFP QY
Gene ID:	27304
Uniprot:	O95396
Cellular Location:	Cytoplasm
Calculated MW:	49kDa
Observed MW:	50kDa

Synonyms:	MOCS3, UBA4
Background:	Molybdenum cofactor (MoCo) is necessary for the function of all molybdoenzymes. The protein encoded by this gene adenylates and activates molybdopterin synthase, an enzyme required for biosynthesis of MoCo. This gene contains no introns. A pseudogene of this gene is present on chromosome 14.

UniProt Protein Function:	MOCS3: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions.
UniProt Protein Details:	Protein type:EC 2.8.1.11; EC 2.7.7.80; Ligase; Transferase Chromosomal Location of Human Ortholog: 20q13.13 Cellular Component: cytosol Molecular Function:sulfurtransferase activity; protein binding; thiosulfate sulfurtransferase activity; metal ion binding; URM1 activating enzyme activity; ATP binding; nucleotidyltransferase activity Biological Process: tRNA wobble uridine modification; protein urmylation; vitamin metabolic process; enzyme active site formation via L-cysteine persulfide; protein amino acid adenylylation; Mo-molybdopterin cofactor biosynthetic process; molybdopterin cofactor biosynthetic process; water-soluble vitamin metabolic process
NCBI Summary:	Molybdenum cofactor (MoCo) is necessary for the function of all molybdoenzymes. The protein encoded by this gene adenylates and activates molybdopterin synthase, an enzyme required for biosynthesis of MoCo. This gene contains no introns. A pseudogene of this gene is present on chromosome 14. [provided by RefSeq, Nov 2012]
UniProt Code:	O95396
NCBI GenInfo Identifier:	22001810
NCBI Gene ID:	27304
NCBI Accession:	O95396.1
UniProt Related Accession:	O95396
Molecular Weight:	460
NCBI Full Name:	Adenylyltransferase and sulfurtransferase MOCS3
NCBI Synonym Full Names:	molybdenum cofactor synthesis 3
NCBI Official Symbol:	MOCS3
NCBI Official Synonym Symbols:	UBA4
NCBI Protein Information:	adenylyltransferase and sulfurtransferase MOCS3; MPT synthase sulfurylase; molybdopterin synthase sulfurylase; molybdenum cofactor synthesis protein 3; ubiquitin-like modifier activating enzyme 4; UBA4, ubiquitin-activating enzyme E1 homolog
UniProt Protein Name:	Adenylyltransferase and sulfurtransferase MOCS3
UniProt Synonym Protein Names:	Molybdenum cofactor synthesis protein 3; Molybdopterin synthase sulfurylase; Adenylyltransferase MOCS3; Sulfur carrier protein MOCS2A adenylyltransferase; ; Sulfur carrier protein MOCS2A sulfurtransferase; Sulfurtransferase MOCS3
UniProt Gene Name:	MOCS3
UniProt Entry Name:	MOCS3_HUMAN

	Western blot analysis of extracts of various cell lines, using MOCS3 antibody (CAB7367) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse kidney using MOCS3 antibody (CAB7367) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of A549 cells using MOCS3 antibody (CAB7367). Blue: DAPI for nuclear staining.