Cell Cycle Antibodies 1
Anti-MNAT1 Antibody (CAB1918)
- SKU:
- CAB1918
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-MNAT1 Antibody |
| Antibody SKU: | CAB1918 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IP |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-309 of human MNAT1 (NP_002422.1). |
| Application: | WB IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IP 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | PC-3, 22Rv1, DU 145, 293T, Mouse testis, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-309 of human MNAT1 (NP_002422.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MDDQ GCPR CKTT KYRN PSLK LMVN VCGH TLCE SCVD LLFV RGAG NCPE CGTP LRKS NFRV QLFE DPTV DKEV EIRK KVLK IYNK REED FPSL REYN DFLE EVEE IVFN LTNN VDLD NTKK KMEI YQKE NKDV IQKN KLKL TREQ EELE EALE VERQ ENEQ RRLF IQKE EQLQ QILK RKNK QAFL DELE SSDL PVAL LLAQ HKDR STQL EMQL EKPK PVKP VTFS TGIK MGQH ISLA PIHK LEEA LYEY QPLQ IETY GPHV PELE MLGR LGYL NHVR AASP QDLA GGYT SSLA CHRA LQDA FSGL FWQP S |
| Gene ID: | 4331 |
| Uniprot: | P51948 |
| Cellular Location: | Nucleus |
| Calculated MW: | 31kDa/35kDa |
| Observed MW: | 36kDa |
| Synonyms: | MNAT1, CAP35, MAT1, RNF66, TFB3 |
| Background: | The protein encoded by this gene, along with cyclin H and CDK7, forms the CDK-activating kinase (CAK) enzymatic complex. This complex activates several cyclin-associated kinases and can also associate with TFIIH to activate transcription by RNA polymerase II. Two transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | MNAT1: Stabilizes the cyclin H-CDK7 complex to form a functional CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. |
| UniProt Protein Details: | Protein type:Nuclear receptor co-regulator; Cell cycle regulation; Ubiquitin conjugating system; Ubiquitin ligase Chromosomal Location of Human Ortholog: 14q23 Cellular Component: nucleoplasm; holo TFIIH complex; cytoplasm Molecular Function:RNA polymerase subunit kinase activity; DNA-dependent ATPase activity; protein binding; zinc ion binding; protein N-terminus binding Biological Process: transcription from RNA polymerase II promoter; viral reproduction; positive regulation of viral transcription; positive regulation of smooth muscle cell proliferation; protein amino acid phosphorylation; regulation of gene expression, epigenetic; mRNA capping; negative regulation of gene expression, epigenetic; transcription-coupled nucleotide-excision repair; nucleotide-excision repair, DNA damage removal; protein complex assembly; G2/M transition of mitotic cell cycle; adult heart development; transcription initiation from RNA polymerase II promoter; ventricular system development; transcription from RNA polymerase I promoter; RNA elongation from RNA polymerase I promoter; termination of RNA polymerase I transcription; DNA repair; regulation of transcription from RNA polymerase II promoter; cell proliferation; nucleotide-excision repair; RNA elongation from RNA polymerase II promoter; gene expression; positive regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; regulation of cyclin-dependent protein kinase activity; transcription initiation from RNA polymerase I promoter; response to calcium ion; negative regulation of apoptosis; G1/S transition of mitotic cell cycle |
| NCBI Summary: | The protein encoded by this gene, along with cyclin H and CDK7, forms the CDK-activating kinase (CAK) enzymatic complex. This complex activates several cyclin-associated kinases and can also associate with TFIIH to activate transcription by RNA polymerase II. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] |
| UniProt Code: | P51948 |
| NCBI GenInfo Identifier: | 1708932 |
| NCBI Gene ID: | 4331 |
| NCBI Accession: | P51948.1 |
| UniProt Secondary Accession: | P51948,Q15817, Q6ICQ7, G3V1U8, |
| UniProt Related Accession: | P51948 |
| Molecular Weight: | 309 |
| NCBI Full Name: | CDK-activating kinase assembly factor MAT1 |
| NCBI Synonym Full Names: | MNAT CDK-activating kinase assembly factor 1 |
| NCBI Official Symbol: | MNAT1 |
| NCBI Official Synonym Symbols: | MAT1; TFB3; CAP35; RNF66 |
| NCBI Protein Information: | CDK-activating kinase assembly factor MAT1; RING finger protein 66; RING finger protein MAT1; CDK7/cyclin-H assembly factor; cyclin G1 interacting protein; cyclin-G1-interacting protein; menage a trois 1 (CAK assembly factor); menage a trois homolog 1, cyclin H assembly factor |
| UniProt Protein Name: | CDK-activating kinase assembly factor MAT1 |
| UniProt Synonym Protein Names: | CDK7/cyclin-H assembly factor; Cyclin-G1-interacting protein; Menage a trois; RING finger protein 66; RING finger protein MAT1; p35; p36 |
| Protein Family: | CDK-activating kinase assembly factor |
| UniProt Gene Name: | MNAT1 |
| UniProt Entry Name: | MAT1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MNAT1 antibody (CAB1918) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug MNAT1 antibody (CAB1918). Western blot was performed from the immunoprecipitate using MNAT1 antibody (CAB1918) at a dilition of 1:1000. |
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