Cell Biology Antibodies 2
Anti-MMP9 Antibody (CAB11391)
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- SKU:
- CAB11391
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-MMP9 Antibody |
| Antibody SKU: | CAB11391 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | IHC |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 400-500 of human MMP9 (NP_004985.2). |
| Application: | IHC |
| Recommended Dilution: | IHC 1:100 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | Mouse brain |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 400-500 of human MMP9 (NP_004985.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | AHEF GHAL GLDH SSVP EALM YPMY RFTE GPPL HKDD VNGI RHLY GPRP EPEP RPPT TTTP QPTA PPTV CPTG PPTV HPSE RPTA GPTG PPSA GPTG PPTA G |
| Gene ID: | 4318 |
| Uniprot: | P14780 |
| Cellular Location: | Secreted, extracellular matrix, extracellular space |
| Calculated MW: | 78kDa |
| Observed MW: | 67kDa |
| Synonyms: | MMP9, CLG4B, GELB, MANDP2, MMP-9 |
| Background: | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. |
| UniProt Protein Function: | MMP9: May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. Exists as monomer or homodimer; disulfide-linked. Exists also as heterodimer with a 25 kDa protein. Macrophages and transformed cell lines produce only the monomeric form. Interacts with ECM1. Activated by 4-aminophenylmercuric acetate and phorbol ester. Up-regulated by ARHGEF4, SPATA13 and APC via the JNK signaling pathway in colorectal tumor cells. Produced by normal alveolar macrophages and granulocytes. Inhibited by histatin-3 1/24 (histatin-5). Inhibited by ECM1. Belongs to the peptidase M10A family. |
| UniProt Protein Details: | Protein type:Protease; Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis; EC 3.4.24.35 Chromosomal Location of Human Ortholog: 20q13.12 Cellular Component: extracellular space; proteinaceous extracellular matrix; extracellular region Molecular Function:collagen binding; identical protein binding; protein binding; zinc ion binding; metalloendopeptidase activity; endopeptidase activity Biological Process: positive regulation of keratinocyte migration; extracellular matrix disassembly; collagen catabolic process; axon guidance; extracellular matrix organization and biogenesis; ossification; macrophage differentiation; positive regulation of apoptosis; ephrin receptor signaling pathway; proteolysis; leukocyte migration; skeletal development; embryo implantation Disease: Metaphyseal Anadysplasia 2 |
| NCBI Summary: | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P14780 |
| NCBI GenInfo Identifier: | 269849668 |
| NCBI Gene ID: | 4318 |
| NCBI Accession: | P14780.3 |
| UniProt Secondary Accession: | P14780,Q3LR70, Q8N725, Q9H4Z1, Q9UCJ9, Q9UCL1, Q9UDK2 B2R7V9, |
| UniProt Related Accession: | P14780 |
| Molecular Weight: | 78,458 Da |
| NCBI Full Name: | Matrix metalloproteinase-9 |
| NCBI Synonym Full Names: | matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) |
| NCBI Official Symbol: | MMP9 |
| NCBI Official Synonym Symbols: | GELB; CLG4B; MMP-9; MANDP2 |
| NCBI Protein Information: | matrix metalloproteinase-9; 92 kDa gelatinase; type V collagenase; macrophage gelatinase; 92 kDa type IV collagenase; matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) |
| UniProt Protein Name: | Matrix metalloproteinase-9 |
| UniProt Synonym Protein Names: | 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB |
| Protein Family: | Matrix metalloproteinase |
| UniProt Gene Name: | MMP9 |
| UniProt Entry Name: | MMP9_HUMAN |
 | Western blot analysis of extracts of mouse brain, using MMP9 antibody (CAB11391) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human stomach using MMP9 antibody (CAB11391) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using MMP9 antibody (CAB11391) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse kidney using MMP9 antibody (CAB11391) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse stomach using MMP9 antibody (CAB11391) at dilution of 1:100 (40x lens). |
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