Cell Biology Antibodies 8

Anti-MMP8 Antibody (CAB1963)

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SKU:
CAB1963
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-MMP8 Antibody
Antibody SKU:CAB1963
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 101-467 of human MMP8 (NP_002415.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:A-549, HL-60, HepG2, Jurkat, MCF7, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 101-467 of human MMP8 (NP_002415.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LTPG NPKW ERTN LTYR IRNY TPQL SEAE VERA IKDA FELW SVAS PLIF TRIS QGEA DINI AFYQ RDHG DNSP FDGP NGIL AHAF QPGQ GIGG DAHF DAEE TWTN TSAN YNLF LVAA HEFG HSLG LAHS SDPG ALMY PNYA FRET SNYS LPQD DIDG IQAI YGLS SNPI QPTG PSTP KPCD PSLT FDAI TTLR GEIL FFKD RYFW RRHP QLQR VEMN FISL FWPS LPTG IQAA YEDF DRDL IFLF KGNQ YWAL SGYD ILQG YPKD ISNY GFPS SVQA IDAA VFYR SKTY FFVN DQFW RYDN QRQF MEPG YPKS ISGA FPGI ESKV DAVF QQEH FFHV FSGP RYYA FDLI AQRV TRVA RGNK WLNC RYG
Gene ID:4317
Uniprot:P22894
Cellular Location:Cytoplasmic granule, Secreted, extracellular matrix, extracellular space
Calculated MW:53kDa
Observed MW:65kDa
Synonyms:MMP8, CLG1, HNC, MMP-8, PMNL-CL
Background:This gene encodes a member of the matrix metalloproteinase (MMP) family of proteins. These proteins are involved in the breakdown of extracellular matrix in embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Proteolysis at different sites on this protein results in multiple active forms of the enzyme with distinct N-termini. This protein functions in the degradation of type I, II and III collagens. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:MMP8: Can degrade fibrillar type I, II, and III collagens. Belongs to the peptidase M10A family.
UniProt Protein Details:

Protein type:Protease; EC 3.4.24.34; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: extracellular space; proteinaceous extracellular matrix; extracellular region

Molecular Function:zinc ion binding; serine-type endopeptidase activity; metalloendopeptidase activity; calcium ion binding

Biological Process: extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; proteolysis

NCBI Summary:Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the enzyme encoded by this gene is stored in secondary granules within neutrophils and is activated by autolytic cleavage. Its function is degradation of type I, II and III collagens. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]
UniProt Code:P22894
NCBI GenInfo Identifier:116862
NCBI Gene ID:4317
NCBI Accession:P22894.1
UniProt Secondary Accession:P22894,Q45F99,
UniProt Related Accession:P22894
Molecular Weight:53,412 Da
NCBI Full Name:Neutrophil collagenase
NCBI Synonym Full Names:matrix metallopeptidase 8 (neutrophil collagenase)
NCBI Official Symbol:MMP8  
NCBI Official Synonym Symbols:HNC; CLG1; MMP-8; PMNL-CL  
NCBI Protein Information:neutrophil collagenase; PMNL collagenase; matrix metalloproteinase-8; matrix metalloproteinase 8 (neutrophil collagenase)
UniProt Protein Name:Neutrophil collagenase
UniProt Synonym Protein Names:Matrix metalloproteinase-8; MMP-8; PMNL collagenase; PMNL-CL
Protein Family:Neutrophil collagenase
UniProt Gene Name:MMP8  
UniProt Entry Name:MMP8_HUMAN
Anti-MMP8 Antibody (CAB1963)Western blot analysis of extracts of various cell lines, using MMP8 antibody (CAB1963) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-MMP8 Antibody (CAB1963)Immunohistochemistry of paraffin-embedded human liver cancer using MMP8 Rabbit pAb (CAB1963) at dilution of 1:150 (40x lens).
Anti-MMP8 Antibody (CAB1963)Immunofluorescence analysis of U2OS cells using MMP8 antibody (CAB1963) at dilution of 1:100. Blue: DAPI for nuclear staining.
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