Cell Biology Antibodies 12

Anti-MMP20 Antibody (CAB9788)

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SKU:
CAB9788
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-MMP20 Antibody
Antibody SKU:CAB9788
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 108-310 of human MMP20 (NP_004762.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:1000
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, mouse brain, rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 108-310 of human MMP20 (NP_004762.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:YRLF PGEP KWKK NTLT YRIS KYTP SMSS VEVD KAVE MALQ AWSS AVPL SFVR INSG EADI MISF ENGD HGDS YPFD GPRG TLAH AFAP GEGL GGDT HFDN AEKW TMGT NGFN LFTV AAHE FGHA LGLA HSTD PSAL MYPT YKYK NPYG FHLP KDDV KGIQ ALYG PRKV FLGK PTLP HAPH HKPS IPDL CDSS SSFD AVTM LGK
Gene ID:9313
Uniprot:O60882
Cellular Location:Secreted, extracellular matrix, extracellular space
Calculated MW:54kDa
Observed MW:54kDa
Synonyms:MMP20, AI2A2, MMP-20
Background:Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene degrades amelogenin, the major protein component of dental enamel matrix, and thus thought to play a role in tooth enamel formation. A mutation in this gene, which alters the normal splice pattern and results in premature termination of the encoded protein, has been associated with amelogenesis imperfecta. This gene is part of a cluster of MMP genes located on chromosome 11q22.3.
UniProt Protein Function:MMP20: Degrades amelogenin, the major protein component of the enamel matrix and two of the macromolecules characterizing the cartilage extracellular matrix: aggrecan and the cartilage oligomeric matrix protein (COMP). May play a central role in tooth enamel formation. Defects in MMP20 are the cause of amelogenesis imperfecta hypomaturation type 2A2 (AI2A2). AI2A2 is an autosomal recessive defect of enamel formation. The disorder involves both primary and secondary dentitions. The teeth have a shiny agar jelly appearance and the enamel is softer than normal. Brown pigment is present in middle layers of enamel. Belongs to the peptidase M10A family.
UniProt Protein Details:

Protein type:EC 3.4.24.-; Secreted, signal peptide; Secreted; Protease

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: extracellular region; extracellular space

Molecular Function:metalloendopeptidase activity; protein binding; serine-type endopeptidase activity

Biological Process: collagen catabolic process

Disease: Amelogenesis Imperfecta, Hypomaturation Type, Iia2

NCBI Summary:Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene degrades amelogenin, the major protein component of dental enamel matrix, and thus thought to play a role in tooth enamel formation. A mutation in this gene, which alters the normal splice pattern and results in premature termination of the encoded protein, has been associated with amelogenesis imperfecta. This gene is part of a cluster of MMP genes located on chromosome 11q22.3. [provided by RefSeq, Aug 2011]
UniProt Code:O60882
NCBI GenInfo Identifier:322510116
NCBI Gene ID:9313
NCBI Accession:O60882.3
UniProt Secondary Accession:O60882,Q6DKT9,
UniProt Related Accession:O60882
Molecular Weight:54,387 Da
NCBI Full Name:Matrix metalloproteinase-20
NCBI Synonym Full Names:matrix metallopeptidase 20
NCBI Official Symbol:MMP20  
NCBI Official Synonym Symbols:AI2A2; MMP-20  
NCBI Protein Information:matrix metalloproteinase-20
UniProt Protein Name:Matrix metalloproteinase-20
UniProt Synonym Protein Names:Enamel metalloproteinase; Enamelysin
Protein Family:Matrix metalloproteinase
UniProt Gene Name:MMP20  
UniProt Entry Name:MMP20_HUMAN
Anti-MMP20 Polyclonal Antibody (CAB9788)Western blot analysis of extracts of various cell lines, using MMP20 antibody (CAB9788) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
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