Cell Biology Antibodies 5

Anti-MMEL1 Antibody (CAB15188)

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SKU:
CAB15188
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-MMEL1 Antibody
Antibody SKU:CAB15188
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 350-450 of human MMEL1 (NP_258428.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:293T, OVCAR3
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 350-450 of human MMEL1 (NP_258428.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:NWTL FIQT VLSS VKIK LLPD EEVV VYGI PYLQ NLEN IIDT YSAR TIQN YLVW RLVL DRIG SLSQ RFKD TRVN YRKA LFGT MVEE VRWR ECVG YVNS NMEN A
Gene ID:79258
Uniprot:Q495T6
Cellular Location:Membrane, Secreted, Single-pass type II membrane protein
Calculated MW:69kDa/71kDa/89kDa
Observed MW:99kDa
Synonyms:MMEL1, MMEL2, NEP2, NEPII, NL1, NL2, SEP
Background:The protein encoded by this gene is a member of the neutral endopeptidase (NEP) or membrane metallo-endopeptidase (MME) family. Family members play important roles in pain perception, arterial pressure regulation, phosphate metabolism and homeostasis. This protein is a type II transmembrane protein and is thought to be expressed as a secreted protein. This gene is expressed mainly in testis with weak expression in the brain, kidney, and heart.
UniProt Protein Function:MELL1: Metalloprotease involved in sperm function, possibly by modulating the processes of fertilization and early embryonic development. Degrades a broad variety of small peptides with a preference for peptides shorter than 3 kDa containing neutral bulky aliphatic or aromatic amino acid residues. Shares the same substrate specificity with MME and cleaves peptides at the same amide bond. Belongs to the peptidase M13 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 3.4.24.11; Membrane protein, integral; Protease

Chromosomal Location of Human Ortholog: 1p36.32

NCBI Summary:The protein encoded by this gene is a member of the neutral endopeptidase (NEP) or membrane metallo-endopeptidase (MME) family. Family members play important roles in pain perception, arterial pressure regulation, phosphate metabolism and homeostasis. This protein is a type II transmembrane protein and is thought to be expressed as a secreted protein. This gene is expressed mainly in testis with weak expression in the brain, kidney, and heart. [provided by RefSeq, Jul 2008]
UniProt Code:Q495T6
NCBI GenInfo Identifier:239049391
NCBI Gene ID:79258
NCBI Accession:NP_258428.2
UniProt Secondary Accession:Q495T6,Q495T7, Q495T8, Q5SZS6, Q96PH9, B9DI79,
UniProt Related Accession:Q495T6
Molecular Weight:89.4
NCBI Full Name:membrane metallo-endopeptidase-like 1
NCBI Synonym Full Names:membrane metalloendopeptidase like 1
NCBI Official Symbol:MMEL1  
NCBI Official Synonym Symbols:NL1; NL2; SEP; NEP2; MMEL2; NEPII  
NCBI Protein Information:membrane metallo-endopeptidase-like 1
UniProt Protein Name:Membrane metallo-endopeptidase-like 1
UniProt Synonym Protein Names:Membrane metallo-endopeptidase-like 2; NEP2(m); Neprilysin II; NEPII
Protein Family:NEDD8-specific protease
UniProt Gene Name:MMEL1  
Anti-MMEL1 Antibody (CAB15188)Western blot analysis of extracts of various cell lines, using MMEL1 antibody (CAB15188) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
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