Name: Anti-MLXIPL Antibody (CAB7630)
Brand: Epigenetics & Nuclear Signaling Antibodies 4
SKU: CAB7630
Availability: OutOfStock

Antibody Name:	Anti-MLXIPL Antibody
Antibody SKU:	CAB7630
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 17-175 of human MLXIPL (NP_116569.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	HepG2, 293T, K562, mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 17-175 of human MLXIPL (NP_116569.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	VAPS PDSD SDTD SEDP SLRR SAGG LLRS QVIH SGHF MVSS PHSD SLPR RRDQ EGSV GPSD FGPR SIDP TLTR LFEC LSLA YSGK LVSP KWKN FKGL KLLC RDKI RLNN AIWR AWYI QYVK RRKS PVCG FVTP LQGP EADA HRKP EAVV LEGN YWKR RIE
Gene ID:	51085
Uniprot:	Q9NP71
Cellular Location:	Nucleus
Calculated MW:	62kDa/78kDa/90kDa/92kDa/93kDa
Observed MW:	110kDa

Synonyms:

MLXIPL, CHREBP, MIO, MLX, MONDOB, WBSCR14, WS-bHLH, bHLHd14

Background:

This gene encodes a basic helix-loop-helix leucine zipper transcription factor of the Myc/Max/Mad superfamily. This protein forms a heterodimeric complex and binds and activates, in a glucose-dependent manner, carbohydrate response element (ChoRE) motifs in the promoters of triglyceride synthesis genes. The gene is deleted in Williams-Beuren syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at chromosome 7q11.23. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	ChREBP: Transcriptional repressor. Binds to the canonical and non-canonical E box sequences 5'-CACGTG-3'. Binds DNA as a heterodimer with TCFL4/MLX. Expressed in liver, heart, kidney, cerebellum and intestinal tissues. 6 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transcription factor Chromosomal Location of Human Ortholog: 7q11.23 Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus Molecular Function:DNA binding; protein heterodimerization activity; protein homodimerization activity; transcription factor activity; transcription factor binding Biological Process: anatomical structure morphogenesis; fatty acid homeostasis; glucose homeostasis; glucose mediated signaling; negative regulation of peptidyl-serine phosphorylation; negative regulation of transcription, DNA-dependent; positive regulation of cell proliferation; positive regulation of fatty acid biosynthetic process; positive regulation of glycolysis; positive regulation of lipid biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of transcription from RNA polymerase II promoter Disease: Williams-beuren Syndrome
NCBI Summary:	This gene encodes a basic helix-loop-helix leucine zipper transcription factor of the Myc/Max/Mad superfamily. This protein forms a heterodimeric complex and binds and activates, in a glucose-dependent manner, carbohydrate response element (ChoRE) motifs in the promoters of triglyceride synthesis genes. The gene is deleted in Williams-Beuren syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at chromosome 7q11.23. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
UniProt Code:	Q9NP71
NCBI GenInfo Identifier:	20140871
NCBI Gene ID:	51085
NCBI Accession:	Q9NP71.1
UniProt Secondary Accession:	Q9NP71,Q96E48, Q9BY03, Q9BY04, Q9BY05, Q9BY06, Q9Y2P3 C5HU02, C5HU03, C5HU04,
UniProt Related Accession:	Q9NP71
Molecular Weight:	78,360 Da
NCBI Full Name:	Carbohydrate-responsive element-binding protein
NCBI Synonym Full Names:	MLX interacting protein like
NCBI Official Symbol:	MLXIPL
NCBI Official Synonym Symbols:	MIO; CHREBP; MONDOB; WBSCR14; WS-bHLH; bHLHd14
NCBI Protein Information:	carbohydrate-responsive element-binding protein
UniProt Protein Name:	Carbohydrate-responsive element-binding protein
UniProt Synonym Protein Names:	Class D basic helix-loop-helix protein 14; bHLHd14; MLX interactor; MLX-interacting protein-like; WS basic-helix-loop-helix leucine zipper protein; WS-bHLH; Williams-Beuren syndrome chromosomal region 14 protein
Protein Family:	Carbohydrate-responsive element-binding protein
UniProt Gene Name:	MLXIPL
UniProt Entry Name:	MLXPL_HUMAN

Western blot analysis of extracts of various cell lines, using MLXIPL antibody (CAB7630) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Antibodies

MLXIPL Antibody (PACO10517)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)