Cell Biology Antibodies 10
Anti-MLN Antibody (CAB6388)
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- SKU:
- CAB6388
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-MLN Antibody |
| Antibody SKU: | CAB6388 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 26-115 of human MLN (NP_002409.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse |
| Positive Samples: | MCF7, SGC-7901, HT-29, Mouse stomach, Mouse intestine |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 26-115 of human MLN (NP_002409.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | FVPI FTYG ELQR MQEK ERNK GQKK SLSV WQRS GEEG PVDP AEPI REEE NEMI KLTA PLEI GMRM NSRQ LEKY PATL EGLL SEML PQHA AK |
| Gene ID: | 4295 |
| Uniprot: | P12872 |
| Cellular Location: | Secreted |
| Calculated MW: | 12kDa |
| Observed MW: | 13kDa |
| Synonyms: | MLN, motilin |
| Background: | This gene encodes a small peptide hormone that is secreted by cells of the small intestine to regulate gastrointestinal contractions and motility. Proteolytic processing of the secreted protein produces the mature peptide and a byproduct referred to as motilin-associated peptide (MAP). Three transcript variants encoding different preproprotein isoforms but the same mature peptide have been found for this gene. |
| UniProt Protein Function: | MLN: Plays an important role in the regulation of interdigestive gastrointestinal motility and indirectly causes rhythmic contraction of duodenal and colonic smooth muscle. Belongs to the motilin family. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 6p21.3 Cellular Component: extracellular region Molecular Function:hormone activity; receptor binding Biological Process: G-protein coupled receptor protein signaling pathway; cell-cell signaling |
| NCBI Summary: | This gene encodes a small peptide hormone that is secreted by cells of the small intestine to regulate gastrointestinal contractions and motility. Proteolytic processing of the secreted protein produces the mature peptide and a byproduct referred to as motilin-associated peptide (MAP). Three transcript variants encoding different preproprotein isoforms but the same mature peptide have been found for this gene. [provided by RefSeq, May 2010] |
| UniProt Code: | P12872 |
| NCBI GenInfo Identifier: | 127253 |
| NCBI Gene ID: | 4295 |
| NCBI Accession: | P12872.1 |
| UniProt Secondary Accession: | P12872,Q2M1L2, Q5T975, Q6NSY7, B7ZLR7, E9PDN2, J3KN51 |
| UniProt Related Accession: | P12872 |
| Molecular Weight: | 115 |
| NCBI Full Name: | Promotilin |
| NCBI Synonym Full Names: | motilin |
| NCBI Official Symbol: | MLN |
| NCBI Protein Information: | promotilin; prepromotilin |
| UniProt Protein Name: | Promotilin |
| Protein Family: | Motilin |
| UniProt Gene Name: | MLN |
| UniProt Entry Name: | MOTI_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MLN Antibody (CAB6388) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry of paraffin-embedded human stomach using MLN antibody (CAB6388) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using MLN antibody (CAB6388) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse heart using MLN antibody (CAB6388) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using MLN antibody (CAB6388). Blue: DAPI for nuclear staining. |
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