Cell Biology Antibodies 9

Anti-MLKL Antibody (CAB5579)

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SKU:
CAB5579
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-MLKL Antibody
Antibody SKU:CAB5579
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant protein of mouse MLKL
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:HeLa, Jurkat, HT-1080, NCI-H460
Immunogen:Recombinant protein of mouse MLKL
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:74568
Uniprot:Q9D2Y4
Cellular Location:Cell membrane, Cytoplasm
Calculated MW:53kDa/54kDa
Observed MW:54kDa
Synonyms:MLKL, hMLKL
Background:This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene.
UniProt Protein Function:MLKL: Belongs to the protein kinase superfamily. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; TKL group; TKL-Unique familyCellular Component: membrane; cytoplasm; plasma membraneMolecular Function: transferase activity, transferring phosphorus-containing groups; protein complex binding; nucleotide binding; protein kinase binding; ATP binding; protein kinase activityBiological Process: programmed cell death; protein amino acid phosphorylation
UniProt Protein Details:
NCBI Summary:This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to lack protein kinase activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (Rip3), which is a key signaling molecule in necroptosis pathway. Knockout of this gene in mice showed that it is essential for necroptosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2015]
UniProt Code:Q9D2Y4
NCBI GenInfo Identifier:81916976
NCBI Gene ID:74568
NCBI Accession:Q9D2Y4.1
UniProt Secondary Accession:Q9D2Y4,Q8CIJ5
UniProt Related Accession:Q9D2Y4
Molecular Weight:54kDa
NCBI Full Name:Mixed lineage kinase domain-like protein
NCBI Synonym Full Names:mixed lineage kinase domain-like
NCBI Official Symbol:Mlkl  
NCBI Official Synonym Symbols:9130019I15Rik  
NCBI Protein Information:mixed lineage kinase domain-like protein; mlkl
UniProt Protein Name:Mixed lineage kinase domain-like protein
UniProt Synonym Protein Names:
Protein Family:Mixed lineage kinase domain-like protein
UniProt Gene Name:Mlkl  
UniProt Entry Name:MLKL_MOUSE
Anti-MLKL Antibody (CAB5579)Western blot analysis of extracts of various cell lines, using MLKL antibody (CAB5579) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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