Description
| Antibody Name: | Anti-MLKL Antibody |
| Antibody SKU: | CAB5579 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant protein of mouse MLKL |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HeLa, Jurkat, HT-1080, NCI-H460 |
| Immunogen: | Recombinant protein of mouse MLKL |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 74568 |
| Uniprot: | Q9D2Y4 |
| Cellular Location: | Cell membrane, Cytoplasm |
| Calculated MW: | 53kDa/54kDa |
| Observed MW: | 54kDa |
| Synonyms: | MLKL, hMLKL |
| Background: | This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. |
| UniProt Protein Function: | MLKL: Belongs to the protein kinase superfamily. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; TKL group; TKL-Unique familyCellular Component: membrane; cytoplasm; plasma membraneMolecular Function: transferase activity, transferring phosphorus-containing groups; protein complex binding; nucleotide binding; protein kinase binding; ATP binding; protein kinase activityBiological Process: programmed cell death; protein amino acid phosphorylation |
| UniProt Protein Details: | |
| NCBI Summary: | This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to lack protein kinase activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (Rip3), which is a key signaling molecule in necroptosis pathway. Knockout of this gene in mice showed that it is essential for necroptosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2015] |
| UniProt Code: | Q9D2Y4 |
| NCBI GenInfo Identifier: | 81916976 |
| NCBI Gene ID: | 74568 |
| NCBI Accession: | Q9D2Y4.1 |
| UniProt Secondary Accession: | Q9D2Y4,Q8CIJ5 |
| UniProt Related Accession: | Q9D2Y4 |
| Molecular Weight: | 54kDa |
| NCBI Full Name: | Mixed lineage kinase domain-like protein |
| NCBI Synonym Full Names: | mixed lineage kinase domain-like |
| NCBI Official Symbol: | Mlkl |
| NCBI Official Synonym Symbols: | 9130019I15Rik |
| NCBI Protein Information: | mixed lineage kinase domain-like protein; mlkl |
| UniProt Protein Name: | Mixed lineage kinase domain-like protein |
| UniProt Synonym Protein Names: | |
| Protein Family: | Mixed lineage kinase domain-like protein |
| UniProt Gene Name: | Mlkl |
| UniProt Entry Name: | MLKL_MOUSE |
 | Western blot analysis of extracts of various cell lines, using MLKL antibody (CAB5579) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
View AllClose
Additional Information
Product Type: |
Antibody |
Reactivity: |
Human |
Reactivity: |
Mouse |
Host Species: |
Rabbit |
Isotype: |
IgG |
Antibody Type: |
Polyclonal Antibody |
Research Area: |
Cell Biology |
Related Products
![Anti-MLKL Antibody [KO Validated] (CAB19685)](https://cdn11.bigcommerce.com/s-rd6ounxcu2/images/stencil/590x590/products/55996/61178/anti-mlkl-antibody-ko-validated-cab19685__57855__69522.1706533730.jpg?c=1 "Anti-MLKL Antibody [KO Validated] (CAB19685)")