Cell Cycle Antibodies 1
Anti-MLH1 Antibody (CAB0254)
- SKU:
- CAB0254
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-MLH1 Antibody |
| Antibody SKU: | CAB0254 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 400-600 of human MLH1 (NP_000240.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HeLa, SW480 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 400-600 of human MLH1 (NP_000240.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LSKP LSSQ PQAI VTED KTDI SSGR ARQQ DEEM LELP APAE VAAK NQSL EGDT TKGT SEMS EKRG PTSS NPRK RHRE DSDV EMVE DDSR KEMT AACT PRRR IINL TSVL SLQE EINE QGHE VLRE MLHN HSFV GCVN PQWA LAQH QTKL YLLN TTKL SEEL FYQI LIYD FANF GVLR LSEP APLF DLAM LALD SPES GWTE E |
| Gene ID: | 4292 |
| Uniprot: | P40692 |
| Cellular Location: | Nucleus |
| Calculated MW: | 58kDa/73kDa/84kDa |
| Observed MW: | 95kDa |
| Synonyms: | MLH1, COCA2, FCC2, HNPCC, HNPCC2, hMLH1 |
| Background: | This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+phenotype) found in HNPCC. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described, but their full-length natures have not been determined. |
| UniProt Protein Function: | MLH1: a protein involved in the repair of mismatches in DNA. Binds PMS2 or MLH1 and MLH3. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50- MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with MBD4. Interacts with EXO1. Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2; Turcot syndrome, an autosomal dominant disorder characterized by malignant tumors of the brain; Muir-Torre syndrome (MTS), a rare autosomal dominant disorder characterized by sebaceous neoplasms and visceral malignancy; lobular carcinoma in situ; endometrial cancer; and non-polyposis colorectal cancer. |
| UniProt Protein Details: | Protein type:Tumor suppressor; DNA repair, damage Chromosomal Location of Human Ortholog: 3p21.3 Cellular Component: chiasma; membrane; MutLalpha complex; nucleoplasm; nucleus; synaptonemal complex Molecular Function:ATPase activity; MutSalpha complex binding; protein binding; single-stranded DNA binding Biological Process: mismatch repair; somatic hypermutation of immunoglobulin genes Disease: Colorectal Cancer, Hereditary Nonpolyposis, Type 2; Mismatch Repair Cancer Syndrome; Muir-torre Syndrome |
| NCBI Summary: | This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+phenotype) found in HNPCC. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described, but their full-length natures have not been determined.[provided by RefSeq, Nov 2009] |
| UniProt Code: | P40692 |
| NCBI GenInfo Identifier: | 730028 |
| NCBI Gene ID: | 4292 |
| NCBI Accession: | P40692.1 |
| UniProt Secondary Accession: | P40692,B4DI13, B4DQ11, E9PCU2, |
| UniProt Related Accession: | P40692 |
| Molecular Weight: | 73,837 Da |
| NCBI Full Name: | DNA mismatch repair protein Mlh1 |
| NCBI Synonym Full Names: | mutL homolog 1 |
| NCBI Official Symbol: | MLH1 |
| NCBI Official Synonym Symbols: | FCC2; COCA2; HNPCC; hMLH1; HNPCC2 |
| NCBI Protein Information: | DNA mismatch repair protein Mlh1 |
| UniProt Protein Name: | DNA mismatch repair protein Mlh1 |
| UniProt Synonym Protein Names: | MutL protein homolog 1 |
| Protein Family: | Putative MutL protein |
| UniProt Gene Name: | MLH1 |
| UniProt Entry Name: | MLH1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MLH1 antibody (CAB0254). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
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