Cell Cycle Antibodies 1
Anti-MLF1 Antibody (CAB13329)
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- SKU:
- CAB13329
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-MLF1 Antibody |
| Antibody SKU: | CAB13329 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-268 of human MLF1 (NP_071888.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse lung, Rat brain, Rat lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-268 of human MLF1 (NP_071888.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MFRM LNSS FEDD PFFS ESIL AHRE NMRQ MIRS FSEP FGRD LLSI SDGR GRAH NRRG HNDG EDSL THTD VSSF QTMD QMVS NMRN YMQK LERN FGQL SVDP NGHS FCSS SVMT YSKI GDEP PKVF QAST QTRR APGG IKET RKAM RDSD SGLE KMAI GHHI HDRA HVIK KSKN KKTG DEEV NQEF INMN ESDA HAFD EEWQ SEVL KYKP GRHN LGNT RMRS VGHE NPGS RELK RREK PQQS PAIE HGRR SNVL GDKL HIKG SSVK SNKK |
| Gene ID: | 4291 |
| Uniprot: | P58340 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 23kDa/27kDa/29kDa/30kDa/33kDa |
| Observed MW: | 38kDa |
| Synonyms: | MLF1 |
| Background: | This gene encodes an oncoprotein which is thought to play a role in the phenotypic determination of hemopoetic cells. Translocations between this gene and nucleophosmin have been associated with myelodysplastic syndrome and acute myeloid leukemia. Multiple transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | MLF1: Involved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. Interferes with erythropoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. Suppresses RFWD2/COP1 activity via CSN3 which activates p53 and induces cell cycle arrest. Binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus. A chromosomal aberration involving MLF1 is a cause of myelodysplastic syndrome (MDS). Translocation t(3;5)(q25.1;q34) with NPM1/NPM. Belongs to the MLF family. 5 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cell cycle regulation Chromosomal Location of Human Ortholog: 3q25.1 Cellular Component: cytoplasm; nucleus Molecular Function:DNA binding; protein binding; protein domain specific binding Biological Process: cell cycle arrest; myeloid progenitor cell differentiation; transcription, DNA-dependent Disease: Leukemia, Acute Myeloid |
| NCBI Summary: | This gene encodes an oncoprotein which is thought to play a role in the phenotypic determination of hemopoetic cells. Translocations between this gene and nucleophosmin have been associated with myelodysplastic syndrome and acute myeloid leukemia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2010] |
| UniProt Code: | P58340 |
| NCBI GenInfo Identifier: | 17368170 |
| NCBI Gene ID: | 4291 |
| NCBI Accession: | P58340.1 |
| UniProt Secondary Accession: | P58340,Q2TLE3, Q2TLE5, Q8N8F8, Q96MH1, E9PEU9, |
| UniProt Related Accession: | P58340 |
| Molecular Weight: | 29,095 Da |
| NCBI Full Name: | Myeloid leukemia factor 1 |
| NCBI Synonym Full Names: | myeloid leukemia factor 1 |
| NCBI Official Symbol: | MLF1 |
| NCBI Protein Information: | myeloid leukemia factor 1 |
| UniProt Protein Name: | Myeloid leukemia factor 1 |
| UniProt Synonym Protein Names: | Myelodysplasia-myeloid leukemia factor 1 |
| Protein Family: | Myeloid leukemia factor |
| UniProt Gene Name: | MLF1 |
| UniProt Entry Name: | MLF1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MLF1 antibody (CAB13329) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of A549 cells using MLF1 antibody (CAB13329). |
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