Cell Biology Antibodies 2
Anti-MKLN1 Antibody (CAB12116)
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- SKU:
- CAB12116
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-MKLN1 Antibody |
| Antibody SKU: | CAB12116 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 436-735 of human MKLN1 (NP_037387.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:1000 |
| Reactivity: | Mouse |
| Positive Samples: | Mouse spleen |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 436-735 of human MKLN1 (NP_037387.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | QTWK LLRE DSCN AGPE DIQS RIGH CMLF HSKN RCLY VFGG QRSK TYLN DFFS YDVD SDHV DIIS DGTK KDSG MVPM TGFT QRAT IDPE LNEI HVLS GLSK DKEK REEN VRNS FWIY DIVR NSWS CVYK NDQA AKDN PTKS LQEE EPCP RFAH QLVY DELH KVHY LFGG NPGK SCSP KMRL DDFW SLKL CRPS KDYL LRHC KYLI RKHR FEEK AQVD PLSA LKYL QNDL YITV DHSD PEET KEFQ LLAS ALFK SGSD FTAL GFSD VDHT YAQR TQLF DTLV NFFP DSMT PPKG NLVD LITL |
| Gene ID: | 4289 |
| Uniprot: | Q9UL63 |
| Cellular Location: | Cell projection, Cytoplasm, cell cortex, ruffle |
| Calculated MW: | 60kDa/84kDa |
| Observed MW: | 100kDa |
| Synonyms: | MKLN1, TWA2, muskelin |
| Background: |
| UniProt Protein Function: | MKLN1: Acts as a mediator of cell spreading and cytoskeletal responses to the extracellular matrix component THBS1. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Cell adhesion Chromosomal Location of Human Ortholog: 7q32 Cellular Component: cell cortex; cytosol; ruffle Biological Process: actin cytoskeleton reorganization; cell-matrix adhesion; regulation of cell shape |
| NCBI Summary: | Muskelin is an intracellular protein that acts as a mediator of cell spreading and cytoskeletal responses to the extracellular matrix component thrombospondin I (MIM 188060) (Adams et al., 1998 [PubMed 9724633]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q9UL63 |
| NCBI GenInfo Identifier: | 23821862 |
| NCBI Gene ID: | 4289 |
| NCBI Accession: | Q9UL63.2 |
| UniProt Secondary Accession: | Q9UL63,Q9NSK4, Q9NUS8, A4D1M8, A6NG43, |
| UniProt Related Accession: | Q9UL63 |
| Molecular Weight: | 60,739 Da |
| NCBI Full Name: | Muskelin |
| NCBI Synonym Full Names: | muskelin 1 |
| NCBI Official Symbol: | MKLN1 |
| NCBI Official Synonym Symbols: | TWA2 |
| NCBI Protein Information: | muskelin |
| UniProt Protein Name: | Muskelin |
| Protein Family: | Muskelin |
| UniProt Gene Name: | MKLN1 |
| UniProt Entry Name: | MKLN1_HUMAN |
 | Western blot analysis of extracts of mouse spleen, using MKLN1 antibody (CAB12116) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
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