Epigenetics & Nuclear Signaling Antibodies 1
Anti-MITF Antibody (CAB1255)
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- SKU:
- CAB1255
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-MITF Antibody |
| Antibody SKU: | CAB1255 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human MITF (NP_937801.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | Jurkat, HeLa, K-562, Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human MITF (NP_937801.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MLEM LEYN HYQV QTHL ENPT KYHI QQAQ RQQV KQYL STTL ANKH ANQV LSLP CPNQ PGDH VMPP VPGS SAPN SPMA MLTL NSNC EKEG FYKF EEQN RAES ECPG MNTH SRAS CMQM DDVI DDII SLES SYNE EILG LMDP ALQM ANTL PVSG NLID LYGN QGLP PPGL TISN SCPA NLPN IKRE LTES EARA LAKE RQKK DNHN LIER RRRF NIND RIKE LGTL IPKS NDPD MRWN KGTI LKAS VDYI RKLQ REQQ RAKE LENR QKKL EHAN RHLL LRIQ |
| Gene ID: | 4286 |
| Uniprot: | O75030 |
| Cellular Location: | Nucleus |
| Calculated MW: | 40kDa/46kDa/52kDa/55kDa/56kDa/57kDa/58kDa |
| Observed MW: | 65kDa |
| Synonyms: | MITF, CMM8, COMMAD, MI, WS2, WS2A, bHLHe32 |
| Background: | This gene encodes a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. |
| UniProt Protein Function: | MITF: a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. Plays a critical role in the differentiation of various cell types including neural crest- derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium. Two isoforms are known: the M-isoform is expressed exclusively in melanocytes, while the A-isoform has a much broader range of expression. Mutations in MITF can lead to Waardenburg syndrome. Ten alternatively spliced isoforms have been described. |
| UniProt Protein Details: | Protein type:Transcription factor; Oncoprotein; DNA-binding Chromosomal Location of Human Ortholog: 3p14.2-p14.1 Cellular Component: protein complex; nucleus Molecular Function:RNA polymerase II transcription factor activity, enhancer binding; protein dimerization activity; protein binding; chromatin binding Biological Process: transcription from RNA polymerase II promoter; regulation of osteoclast differentiation; camera-type eye development; cell fate commitment; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; melanocyte differentiation; protein complex assembly; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; osteoclast differentiation; bone remodeling; regulation of cell proliferation Disease: Waardenburg Syndrome, Type 2a; Albinism, Ocular, With Sensorineural Deafness; Melanoma, Cutaneous Malignant, Susceptibility To, 8; Tietz Syndrome |
| NCBI Summary: | This gene encodes a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O75030 |
| NCBI GenInfo Identifier: | 13124344 |
| NCBI Gene ID: | 4286 |
| NCBI Accession: | O75030.2 |
| UniProt Secondary Accession: | O75030,Q14841, Q9P2V0, Q9P2V1, Q9P2V2, Q9P2Y8, B4DJL2 D3K197, E9PFN0, |
| UniProt Related Accession: | O75030 |
| Molecular Weight: | 526 |
| NCBI Full Name: | Microphthalmia-associated transcription factor |
| NCBI Synonym Full Names: | microphthalmia-associated transcription factor |
| NCBI Official Symbol: | MITF |
| NCBI Official Synonym Symbols: | MI; WS2; CMM8; WS2A; bHLHe32 |
| NCBI Protein Information: | microphthalmia-associated transcription factor; class E basic helix-loop-helix protein 32 |
| UniProt Protein Name: | Microphthalmia-associated transcription factor |
| UniProt Synonym Protein Names: | Class E basic helix-loop-helix protein 32; bHLHe32 |
| Protein Family: | Microphthalmia-associated transcription factor |
| UniProt Gene Name: | MITF |
| UniProt Entry Name: | MITF_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MITF antibody (CAB1255) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
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