Cell Cycle Antibodies 1

Anti-MIS12 Antibody (CAB15508)

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SKU:
CAB15508
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-MIS12 Antibody
Antibody SKU:CAB15508
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-205 of human MIS12 (NP_076944.1).
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Human
Positive Samples:HeLa, 293T, LO2
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-205 of human MIS12 (NP_076944.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSVD PMTY EAQF FGFT PQTC MLRI YIAF QDYL FEVM QAVE QVIL KKLD GIPD CDIS PVQI RKCT EKFL CFMK GHFD NLFS KMEQ LFLQ LILR IPSN ILLP EDKC KETP YSEE DFQH LQKE IEQL QEKY KTEL CTKQ ALLA ELEE QKIV QAKL KQTL TFFD ELHN VGRD HGTS DFRE SLVS LVQN SRKL QNIR DNVE KESK RLKI S
Gene ID:79003
Uniprot:Q9H081
Cellular Location:Chromosome, Nucleus, centromere, kinetochore
Calculated MW:24kDa
Observed MW:24kDa
Synonyms:MIS12, 2510025F08Rik, KNTC2AP, MTW1, hMis12
Background:
UniProt Protein Function:MIS12: Part of the MIS12 complex which is required for normal chromosome alignment and segregation and for kinetochore formation during mitosis. Belongs to the mis12 family.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 17p13.2

Cellular Component: nucleus; cytosol; MIS12/MIND type complex

Molecular Function:protein binding

Biological Process: mitosis; kinetochore assembly; cell division; mitotic cell cycle; chromosome segregation

UniProt Code:Q9H081
NCBI GenInfo Identifier:384551679
NCBI Gene ID:79003
NCBI Accession:NP_076944.1
UniProt Secondary Accession:Q9H081,Q96N24,
UniProt Related Accession:Q9H081
Molecular Weight:24,140 Da
NCBI Full Name:Homo sapiens MIS12 kinetochore complex component (MIS12), transcript variant 2, mRNA
NCBI Synonym Full Names:MIS12 kinetochore complex component
NCBI Official Symbol:MIS12  
NCBI Official Synonym Symbols:MTW1; hMis12; KNTC2AP; 2510025F08Rik  
NCBI Protein Information:protein MIS12 homolog; MIS12, MIND kinetochore complex component, homolog; homolog of yeast Mis12
UniProt Protein Name:Protein MIS12 homolog
Protein Family:Protein
UniProt Gene Name:MIS12  
UniProt Entry Name:MIS12_HUMAN
Anti-MIS12 Antibody (CAB15508)Western blot analysis of extracts of various cell lines, using MIS12 antibody (CAB15508) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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