Antibody Name:	Anti-MIP Antibody
Antibody SKU:	CAB2886
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 50-150 of human MIP (NP_036196.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:1000 IHC 1:100 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, U-251MG, Mouse skeletal muscle, Mouse heart, Mouse liver, Mouse testis, Mouse eye, Rat eye

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 50-150 of human MIP (NP_036196.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LALA TLVQ SVGH ISGA HVNP AVTF AFLV GSQM SLLR AFCY MAAQ LLGA VAGA AVLY SVTP PAVR GNLA LNTL HPAV SVGQ ATTV EIFL TLQF VLCI FATY D
Gene ID:	4284
Uniprot:	P30301
Cellular Location:	Cell junction, Cell membrane, Multi-pass membrane protein, gap junction
Calculated MW:	28kDa
Observed MW:	28kDa

Synonyms:

MIP, AQP0, CTRCT15, LIM1, MIP26, MP26

Background:

Major intrinsic protein is a member of the water-transporting aquaporins as well as the original member of the MIP family of channel proteins. The function of the fiber cell membrane protein encoded by this gene is undetermined, yet this protein is speculated to play a role in intracellular communication. The MIP protein is expressed in the ocular lens and is required for correct lens function. This gene has been mapped among aquaporins AQP2, AQP5, and AQP6, in a potential gene cluster at 12q13.

UniProt Protein Function:	AQP0: major intrinsic protein of lens fiber. A member of the water-transporting aquaporins as well as the original member of the MIP family of channel proteins. May be responsible for regulating the osmolarity of the lens. Defects in MIP are a cause of autosomal recessive congenital cataract.
UniProt Protein Details:	Protein type:Membrane protein, integral; Membrane protein, multi-pass; Channel, misc.; Transporter, aquaporin family Chromosomal Location of Human Ortholog: 12q13 Cellular Component: intracellular canaliculus; intracellular membrane-bound organelle; integral to plasma membrane; apical plasma membrane; plasma membrane; gap junction Molecular Function:calmodulin binding; transporter activity; water channel activity; structural constituent of eye lens; glycerol channel activity Biological Process: lens development in camera-type eye; glycerol transport; cellular water homeostasis; visual perception; canalicular bile acid transport; water transport; response to stimulus; transmembrane transport Disease: Cataract 15, Multiple Types
NCBI Summary:	Major intrinsic protein is a member of the water-transporting aquaporins as well as the original member of the MIP family of channel proteins. The function of the fiber cell membrane protein encoded by this gene is undetermined, yet this protein is speculated to play a role in intracellular communication. The MIP protein is expressed in the ocular lens and is required for correct lens function. This gene has been mapped among aquaporins AQP2, AQP5, and AQP6, in a potential gene cluster at 12q13. [provided by RefSeq, Jul 2008]
UniProt Code:	P30301
NCBI GenInfo Identifier:	266537
NCBI Gene ID:	4284
NCBI Accession:	P30301.1
UniProt Secondary Accession:	P30301,Q17R41,
UniProt Related Accession:	P30301
Molecular Weight:	263
NCBI Full Name:	Lens fiber major intrinsic protein
NCBI Synonym Full Names:	major intrinsic protein of lens fiber
NCBI Official Symbol:	MIP
NCBI Official Synonym Symbols:	AQP0; LIM1; MP26; MIP26; CTRCT15
NCBI Protein Information:	lens fiber major intrinsic protein; aquaporin 0
UniProt Protein Name:	Lens fiber major intrinsic protein
UniProt Synonym Protein Names:	Aquaporin-0; MIP26; MP26
Protein Family:	Mipartoxin
UniProt Gene Name:	MIP
UniProt Entry Name:	MIP_HUMAN

	Western blot analysis of extracts of various cell lines, using MIP antibody (CAB2886) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse brain using MIP antibody (CAB2886) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using MIP antibody (CAB2886) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using MIP antibody (CAB2886) at dilution of 1:100 (40x lens).