Anti-Mineralocorticoid receptor (NR3C2) Antibody (CAB21058)

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SKU:
CAB21058

Description

Antibody Name:Anti-Mineralocorticoid receptor (NR3C2) Antibody (CAB21058)
Antibody SKU:CAB21058
Antibody Size:50µL, 100µL
Application:Western blotting
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 450-600 of human Mineralocorticoid receptor (NR3C2) (NP_000892.2).
Application:Western blotting
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:PC-3, THP-1
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 450-600 of human Mineralocorticoid receptor (NR3C2) (NP_000892.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:FPFM DGSY FSFM DDKD YYSL SGIL GPPV PGFD GNCE GSGF PVGI KQEP DDGS YYPE ASIP SSAI VGVN SGGQ SFHY RIGA QGTI SLSR SARD QSFQ HLSS FPPV NTLV ESWK SHGD LSSR RSDG YPVL EYIP ENVS SSTL RSVS TGSS RPS
Cellular Location:Cytoplasm, Endoplasmic reticulum membrane, Nucleus, Peripheral membrane protein
Calculated MW:75kDa/94kDa/107kDa
Observed MW:100KDa
Synonyms:NR3C2, MCR, MLR, MR, NR3C2VIT
Background:This gene encodes the mineralocorticoid receptor, which mediates aldosterone actions on salt and water balance within restricted target cells. The protein functions as a ligand-dependent transcription factor that binds to mineralocorticoid response elements in order to transactivate target genes. Mutations in this gene cause autosomal dominant pseudohypoaldosteronism type I, a disorder characterized by urinary salt wasting. Defects in this gene are also associated with early onset hypertension with severe exacerbation in pregnancy. Alternative splicing results in multiple transcript variants.
Western blot analysis of extracts of various cell lines, using Mineralocorticoid receptor (NR3C2) antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.Western blot analysis of extracts of various cell lines, using Mineralocorticoid receptor (NR3C2) antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
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Additional Information

Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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