Immunology Antibodies 1
Anti-MIF Antibody (CAB1391)
- SKU:
- CAB1391
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-MIF Antibody |
| Antibody SKU: | CAB1391 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-115 of human MIF (NP_002406.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, HL-60, Mouse liver, Mouse brain, Mouse kidney, Rat liver, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-115 of human MIF (NP_002406.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MPMF IVNT NVPR ASVP DGFL SELT QQLA QATG KPPQ YIAV HVVP DQLM AFGG SSEP CALC SLHS IGKI GGAQ NRSY SKLL CGLL AERL RISP DRVY INYY DMNA ANVG WNNS TFA |
| Gene ID: | 4282 |
| Uniprot: | P14174 |
| Cellular Location: | Cytoplasm, Secreted |
| Calculated MW: | 12kDa |
| Observed MW: | 12KDa |
| Synonyms: | MIF, GIF, GLIF, MMIF |
| Background: | This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. |
| UniProt Protein Function: | MIF: a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. Also acts as a phenylpyruvate tautomerase. |
| UniProt Protein Details: | Protein type:EC 5.3.2.1; Isomerase; EC 5.3.3.12; Amino Acid Metabolism - tyrosine; Cell development/differentiation; Cytokine; Apoptosis; Amino Acid Metabolism - phenylalanine Chromosomal Location of Human Ortholog: 22q11.23 Cellular Component: cell surface; cytoplasm; extracellular region; extracellular space; myelin sheath; nucleoplasm; vesicle Molecular Function:chemoattractant activity; cytokine activity; dopachrome isomerase activity; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; phenylpyruvate tautomerase activity; protein binding; receptor binding Biological Process: blood coagulation; carboxylic acid metabolic process; cell aging; cell proliferation; cell surface receptor linked signal transduction; DNA damage response, signal transduction by p53 class mediator; inflammatory response; innate immune response; leukocyte migration; negative regulation of apoptosis; negative regulation of cellular protein metabolic process; negative regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of mature B cell apoptosis; negative regulation of myeloid cell apoptosis; positive chemotaxis; positive regulation of B cell proliferation; positive regulation of cytokine secretion; positive regulation of fibroblast proliferation; positive regulation of lipopolysaccharide-mediated signaling pathway; positive regulation of MAP kinase activity; positive regulation of peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphorylation; prostaglandin biosynthetic process; regulation of macrophage activation Disease: Rheumatoid Arthritis, Systemic Juvenile |
| NCBI Summary: | This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P14174 |
| NCBI GenInfo Identifier: | 1170955 |
| NCBI Gene ID: | 4282 |
| NCBI Accession: | P14174.4 |
| UniProt Secondary Accession: | P14174,Q2V4Y5, Q6FHV0, A5Z1R8, B2R4S3, |
| UniProt Related Accession: | P14174 |
| Molecular Weight: | 12,476 Da |
| NCBI Full Name: | Macrophage migration inhibitory factor |
| NCBI Synonym Full Names: | macrophage migration inhibitory factor (glycosylation-inhibiting factor) |
| NCBI Official Symbol: | MIF |
| NCBI Official Synonym Symbols: | GIF; GLIF; MMIF |
| NCBI Protein Information: | macrophage migration inhibitory factor |
| UniProt Protein Name: | Macrophage migration inhibitory factor |
| UniProt Synonym Protein Names: | Glycosylation-inhibiting factor; GIF; L-dopachrome isomerase; L-dopachrome tautomerase (EC:5.3.3.12); Phenylpyruvate tautomerase |
| Protein Family: | MIF-like protein |
| UniProt Gene Name: | MIF |
| UniProt Entry Name: | MIF_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MIF antibody (CAB1391) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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