Cell Biology Antibodies 1
Anti-MIEF1 Antibody (CAB10396)
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- SKU:
- CAB10396
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-MIEF1 Antibody |
| Antibody SKU: | CAB10396 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 40-280 of human MIEF1 (NP_061881.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | 293T, U-87MG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 40-280 of human MIEF1 (NP_061881.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | GIAT LAVK RMYD RAIS APTS PTRL SHSG KRSW EEPN WMGS PRLL NRDM KTGL SRSL QTLP TDSS TFDT DTFC PPRP KPVA RKGQ VDLK KSRL RMSL QEKL LTYY RNRA AIPA GEQA RAKQ AAVD ICAE LRSF LRAK LPDM PLRD MYLS GSLY DDLQ VVTA DHIQ LIVP LVLE QNLW SCIP GEDT IMNV PGFF LVRR ENPE YFPR GSSY WDRC VVGG YLSP KTVA DTFE KVVA GSIN WPAI G |
| Gene ID: | 54471 |
| Uniprot: | Q9NQG6 |
| Cellular Location: | Mitochondrion outer membrane, Single-pass membrane protein |
| Calculated MW: | 8kDa/15kDa/51kDa |
| Observed MW: | 51kDa |
| Synonyms: | MIEF1, HSU79252, MID51, SMCR7L, dJ1104E15.3 |
| Background: |
| UniProt Protein Function: | SMCR7L: Mitochondrial outer membrane protein which regulates mitochondrial morphology. Mitochondrial morphology is controlled by two opposites processes: fusion and fission. Seems to have a key role in inhibiting DNM1L-induced mitochondrial fission. Belongs to the SMCR7 family. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 22q13 Cellular Component: mitochondrial outer membrane; mitochondrion; integral to membrane; peroxisome Molecular Function:identical protein binding; protein binding; GDP binding; ADP binding Biological Process: mitochondrial fission; mitochondrial fusion |
| UniProt Code: | Q9NQG6 |
| NCBI GenInfo Identifier: | 74752902 |
| NCBI Gene ID: | 54471 |
| NCBI Accession: | Q9NQG6.1 |
| UniProt Secondary Accession: | Q9NQG6,Q7L890, Q9BUI3, |
| UniProt Related Accession: | Q9NQG6 |
| Molecular Weight: | Calculated MW: 8kDa/15kDa/51kDaObserved MW: 51kDa |
| NCBI Full Name: | Mitochondrial dynamics protein MID51 |
| NCBI Synonym Full Names: | mitochondrial elongation factor 1 |
| NCBI Official Symbol: | MIEF1 |
| NCBI Official Synonym Symbols: | MID51; SMCR7L; HSU79252; dJ1104E15.3 |
| NCBI Protein Information: | mitochondrial dynamics protein MID51; SMCR7-like protein; mitochondrial dynamic protein MID51; mitochondrial dynamic protein of 51 kDa; mitochondrial dynamics protein of 51 kDa; Smith-Magenis syndrome chromosome region, candidate 7-like; smith-Magenis syndrome chromosomal region candidate gene 7 protein-like |
| UniProt Protein Name: | Mitochondrial dynamics protein MID51 |
| UniProt Synonym Protein Names: | Mitochondrial dynamics protein of 51 kDa; Mitochondrial elongation factor 1; Smith-Magenis syndrome chromosomal region candidate gene 7 protein-like; SMCR7-like protein |
| Protein Family: | Mitochondrial dynamics protein |
| UniProt Gene Name: | MIEF1 |
| UniProt Entry Name: | MID51_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MIEF1 antibody (CAB10396) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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