Name: Anti-MID2 Antibody (CAB8325)
Brand: Signal Transduction Antibodies 3
SKU: CAB8325
Availability: OutOfStock

Antibody Name:	Anti-MID2 Antibody
Antibody SKU:	CAB8325
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 476-705 of human MID2 (NP_438112.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	BT-474, 293T, U-251MG, Mouse lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 476-705 of human MID2 (NP_438112.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GTRY IFIV KAIN QAGS RNSE PTRL KTNS QPFK LDPK MTHK KLKI SNDG LQME KDES SLKK SHTP ERFS GTGC YGAA GNIF IDSG CHYW EVVM GSST WYAI GIAY KSAP KNEW IGKN ASSW VFSR CNSN FVVR HNNK EMLV DVPP HLKR LGVL LDYD NNML SFYD PANS LHLH TFDV TFIL PVCP TFTI WNKS LMIL SGLP APDF IDYP ERQE CNCR PQES PYVS GMKT CH
Gene ID:	11043
Uniprot:	Q9UJV3
Cellular Location:	Cytoplasm, cytoskeleton
Calculated MW:	79kDa/83kDa
Observed MW:	83kDa

Synonyms:	MID2, FXY2, MRX101, RNF60, TRIM1, midline 2
Background:	The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of this gene results in two transcript variants encoding different isoforms.

UniProt Protein Function:	MID2: is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Feb 2009]
UniProt Protein Details:	Protein type:Microtubule-binding; EC 6.3.2.-; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: Xq22.3 Cellular Component: microtubule Molecular Function:microtubule binding; phosphoprotein binding; protein heterodimerization activity; protein homodimerization activity Biological Process: activation of NF-kappaB transcription factor; innate immune response; negative regulation of viral transcription; negative regulation of virion penetration into host cell; positive regulation of autophagy; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of transcription factor activity Disease: Mental Retardation, X-linked 101
NCBI Summary:	The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Feb 2009]
UniProt Code:	Q9UJV3
NCBI GenInfo Identifier:	294862489
NCBI Gene ID:	11043
NCBI Accession:	Q9UJV3.3
UniProt Secondary Accession:	Q9UJV3,Q5JYF5, Q8WWK1, Q9UJR9, A6NEL8, A6PVI5,
UniProt Related Accession:	Q9UJV3
Molecular Weight:	79,865 Da
NCBI Full Name:	Probable E3 ubiquitin-protein ligase MID2
NCBI Synonym Full Names:	midline 2
NCBI Official Symbol:	MID2
NCBI Official Synonym Symbols:	FXY2; RNF60; TRIM1; MRX101
NCBI Protein Information:	probable E3 ubiquitin-protein ligase MID2
UniProt Protein Name:	Probable E3 ubiquitin-protein ligase MID2
UniProt Synonym Protein Names:	Midin-2; Midline defect 2; Midline-2; RING finger protein 60; Tripartite motif-containing protein 1
Protein Family:	Cell wall integrity sensor
UniProt Gene Name:	MID2
UniProt Entry Name:	TRIM1_HUMAN

Western blot analysis of extracts of various cell lines, using MID2 antibody (CAB8325) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 40s.

Antibodies

MID2 Antibody (PACO10490)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)