Antibody Name:	Anti-MID1 Antibody
Antibody SKU:	CAB7291
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 478-667 of human MID1 (NP_000372.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 478-667 of human MID1 (NP_000372.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KLKT NSQP FKLD PKSA HRKL KVSH DNLT VERD ESSS KKSH TPER FTSQ GSYG VAGN VFID SGRH YWEV VISG STWY AIGL AYKS APKH EWIG KNSA SWAL CRCN NNWV VRHN SKEI PIEP APHL RRVG ILLD YDNG SIAF YDAL NSIH LYTF DVAF AQPV CPTF TVWN KCLT IITG LPIP DHLD CTEQ LP
Gene ID:	4281
Uniprot:	O15344
Cellular Location:	Cytoplasm, cytoskeleton, spindle
Calculated MW:	62kDa/75kDa
Observed MW:	75KDa

Synonyms:

MID1, BBBG1, FXY, GBBB1, MIDIN, OGS1, OS, OSX, RNF59, TRIM18, XPRF, ZNFXY, midline 1

Background:

The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also known as the 'RING-B box-coiled coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein forms homodimers which associate with microtubules in the cytoplasm. The protein is likely involved in the formation of multiprotein structures acting as anchor points to microtubules. Mutations in this gene have been associated with the X-linked form of Opitz syndrome, which is characterized by midline abnormalities such as cleft lip, laryngeal cleft, heart defects, hypospadias, and agenesis of the corpus callosum. This gene was also the first example of a gene subject to X inactivation in human while escaping it in mouse. Alternative promoter use, alternative splicing and alternative polyadenylation result in multiple transcript variants that have different tissue specificities.

UniProt Protein Function:	MID1: Has E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which results in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by polyubiquitination. Defects in MID1 are the cause of Opitz GBBB syndrome 1 (OGS1). A congenital midline malformation syndrome characterized by hypertelorism, genital-urinary defects such as hypospadias in males and splayed labia in females, lip-palate- laryngotracheal clefts, imperforate anus, developmental delay and congenital heart defects. MID1 mutations produce proteins with a decreased affinity for microtubules. Belongs to the TRIM/RBCC family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Ligase; EC 6.3.2.-; Cytoskeletal; Ubiquitin conjugating system; EC 6.3.2.19; Ubiquitin ligase Chromosomal Location of Human Ortholog: Xp22 Cellular Component: microtubule; microtubule associated complex; cytoplasmic microtubule; spindle; cytosol Molecular Function:protein homodimerization activity; zinc ion binding; protein heterodimerization activity; microtubule binding; ubiquitin protein ligase binding; phosphoprotein binding; ligase activity Biological Process: metabolic process; negative regulation of microtubule depolymerization; cytokine and chemokine mediated signaling pathway; microtubule cytoskeleton organization and biogenesis; pattern specification process; positive regulation of stress-activated MAPK cascade Disease: Tracheoesophageal Fistula With Or Without Esophageal Atresia; Opitz Gbbb Syndrome, X-linked
NCBI Summary:	The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also known as the 'RING-B box-coiled coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein forms homodimers which associate with microtubules in the cytoplasm. The protein is likely involved in the formation of multiprotein structures acting as anchor points to microtubules. Mutations in this gene have been associated with the X-linked form of Opitz syndrome, which is characterized by midline abnormalities such as cleft lip, laryngeal cleft, heart defects, hypospadias, and agenesis of the corpus callosum. This gene was also the first example of a gene subject to X inactivation in human while escaping it in mouse. Multiple different transcript variants are generated by alternate splicing; however, the full-length nature of some of the variants has not been determined. [provided by RefSeq, Jul 2010]
UniProt Code:	O15344
NCBI GenInfo Identifier:	22653810
NCBI Gene ID:	4281
NCBI Accession:	O15344.1
UniProt Secondary Accession:	O15344,O75361, Q9BZX5, B2RCG2,
UniProt Related Accession:	O15344
Molecular Weight:	Calculated MW: 62kDa/75kDaObserved MW: 75-85kDa
NCBI Full Name:	E3 ubiquitin-protein ligase Midline-1
NCBI Synonym Full Names:	midline 1
NCBI Official Symbol:	MID1
NCBI Official Synonym Symbols:	OS; FXY; OSX; OGS1; XPRF; BBBG1; GBBB1; MIDIN; RNF59; ZNFXY; TRIM18
NCBI Protein Information:	E3 ubiquitin-protein ligase Midline-1; Opitz/BBB syndrome; RING finger protein 59; RING finger protein Midline-1; midline 1 RING finger protein; tripartite motif protein TRIM18; putative transcription factor XPRF; tripartite motif-containing protein 18; zinc finger on X and Y, mouse, homolog of
UniProt Protein Name:	E3 ubiquitin-protein ligase Midline-1
UniProt Synonym Protein Names:	Midin; Putative transcription factor XPRF; RING finger protein 59; RING finger protein Midline-1; Tripartite motif-containing protein 18
Protein Family:	Mid1-interacting protein
UniProt Gene Name:	MID1
UniProt Entry Name:	TRI18_HUMAN

	Western blot analysis of extracts of HeLa cells, using MID1 Rabbit pAb (CAB7291) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunofluorescence analysis of MCF-7 cells using MID1 antibody (CAB7291). Blue: DAPI for nuclear staining.