Description
| Antibody Name: | Anti-MICB Antibody |
| Antibody SKU: | CAB9802 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 23-108 of human MICB (NP_005922.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | NCI-H460, SKOV3 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 23-108 of human MICB (NP_005922.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | AEPH SLRY NLMV LSQD GSVQ SGFL AEGH LDGQ PFLR YDRQ KRRA KPQG QWAE NVLG AKTW DTET EDLT ENGQ DLRR TLTH IKDQ KG |
| Gene ID: | 4277 |
| Uniprot: | Q29980 |
| Cellular Location: | Cell membrane, Single-pass type I membrane protein |
| Calculated MW: | 4kDa/37kDa/42kDa |
| Observed MW: | 60kDa |
| Synonyms: | MICB, PERB11.2 |
| Background: | This gene encodes a heavily glycosylated protein which is a ligand for the NKG2D type II receptor. Binding of the ligand activates the cytolytic response of natural killer (NK) cells, CD8 alphabeta T cells, and gammadelta T cells which express the receptor. This protein is stress-induced and is similar to MHC class I molecules; however, it does not associate with beta-2-microglobulin or bind peptides. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | MICB: Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis. Genetic variations in MICA are a cause of susceptibility to rheumatoid arthritis (RA). It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. The MICB*004 allele is associated with rheumatoid arthritis. Genetic variation in MICB is associated with cytomegalovirus and herpes simplex virus I seropositivity and this may be associated with schizophrenia risk. Belongs to the MHC class I family. MIC subfamily. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 6p21.3 Cellular Component: cell surface; plasma membrane; integral to membrane Molecular Function:beta-2-microglobulin binding; antigen binding; natural killer cell lectin-like receptor binding Biological Process: antigen processing and presentation; regulation of immune response; response to retinoic acid; viral reproduction; response to heat; natural killer cell mediated cytotoxicity; cytolysis; immune response-activating cell surface receptor signaling pathway; response to oxidative stress; negative regulation of defense response to virus by host; gamma-delta T cell activation; T cell mediated cytotoxicity |
| NCBI Summary: | This gene encodes a heavily glycosylated protein which is a ligand for the NKG2D type II receptor. Binding of the ligand activates the cytolytic response of natural killer (NK) cells, CD8 alphabeta T cells, and gammadelta T cells which express the receptor. This protein is stress-induced and is similar to MHC class I molecules; however, it does not associate with beta-2-microglobulin or bind peptides. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
| UniProt Code: | Q29980 |
| NCBI GenInfo Identifier: | 74706857 |
| NCBI Gene ID: | 4277 |
| NCBI Accession: | Q29980.1 |
| UniProt Secondary Accession: | Q29980,O14499, O14500, O19798, O19799, O19800, O19801 O19802, A2AC57, A6NP85, B0UZ10, B2RAK2, |
| UniProt Related Accession: | Q29980 |
| Molecular Weight: | 4,287 Da |
| NCBI Full Name: | MHC class I polypeptide-related sequence B |
| NCBI Synonym Full Names: | MHC class I polypeptide-related sequence B |
| NCBI Official Symbol: | MICB |
| NCBI Official Synonym Symbols: | PERB11.2 |
| NCBI Protein Information: | MHC class I polypeptide-related sequence B; MHC class I mic-B antigen; stress inducible class I homolog; MICB {ECO:0000312|EMBL:CAA62823.1}; MHC class I chain-related protein B; MHC class I-like molecule PERB11.2-IMX |
| UniProt Protein Name: | MHC class I polypeptide-related sequence B |
| Protein Family: | MHC class I polypeptide-related sequence |
| UniProt Gene Name: | MICB |
| UniProt Entry Name: | MICB_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MICB antibody (CAB9802) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
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Additional Information
Product Type: |
Antibody |
Applications: |
WB |
Reactivity: |
Human |
Host Species: |
Rabbit |
Isotype: |
IgG |
Research Area: |
Immunology |
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