Cell Biology Antibodies 6
Anti-MICAL1 Antibody (CAB15505)
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- SKU:
- CAB15505
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-MICAL1 Antibody |
| Antibody SKU: | CAB15505 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 718-1067 of human MICAL1 (NP_073602.3). |
| Application: | WB |
| Recommended Dilution: | WB 1:200 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | A-549, HT-29, Raji |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 718-1067 of human MICAL1 (NP_073602.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | HRSC FRCH TCEA TLWP GGYE QHPG DGHF YCLQ HLPQ TDHK AEGS DRGP ESPE LPTP SENS MPPG LSTP TASQ EGAG PVPD PSQP TRRQ IRLS SPER QRLS SLNL TPDP EMEP PPKP PRSC SALA RHAL ESSF VGWG LPVQ SPQA LVAM EKEE KESP FSSE EEEE DVPL DSDV EQAL QTFA KTSG TMNN YPTW RRTL LRRA KEEE MKRF CKAQ TIQR RLNE IEAA LREL EAEG VKLE LALR RQSS SPEQ QKKL WVGQ LLQL VDKK NSLV AEEA ELMI TVQE LNLE EKQW QLDQ ELRG YMNR EENL KTAA DRQA EDQV LRKL VDLV NQRD ALIR FQEE RRLS ELAL GTGA QG |
| Gene ID: | 64780 |
| Uniprot: | Q8TDZ2 |
| Cellular Location: | Cytoplasm, cytoskeleton |
| Calculated MW: | 37kDa/108kDa/117kDa/119kDa |
| Observed MW: | 132kDa |
| Synonyms: | MICAL1, MICAL, MICAL-1, NICAL |
| Background: | This gene encodes an enzyme that oxidizes methionine residues on actin, thereby promoting depolymerization of actin filaments. This protein interacts with and regulates signalling by NEDD9/CAS-L (neural precursor cell expressed, developmentally down-regulated 9). Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | MICAL1: Monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin. Acts by modifying actin subunits through the addition of oxygen to form methionine-sulfoxide, leading to promote actin filament severing and prevent repolymerization (Probable). Acts as a cytoskeletal regulator that connects NEDD9 to intermediate filaments. Also acts as a negative regulator of apoptosis via its interaction with STK38 and STK38L; acts by antagonizing STK38 and STK38L activation by MST1/STK4. Belongs to the Mical family. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Adaptor/scaffold; EC 1.14.13.- Chromosomal Location of Human Ortholog: 6q21 Cellular Component: cytoplasm; intermediate filament Molecular Function:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NADH or NADPH as one donor, and incorporation of one atom of oxygen; protein binding; zinc ion binding; actin binding; SH3 domain binding; Rab GTPase binding Biological Process: actin filament depolymerization; negative regulation of protein amino acid phosphorylation; cytoskeleton organization and biogenesis; negative regulation of caspase activity; signal transduction; sulfur oxidation |
| UniProt Code: | Q8TDZ2 |
| NCBI GenInfo Identifier: | 45593495 |
| NCBI Gene ID: | |
| NCBI Accession: | Q8TDZ2.2 |
| Molecular Weight: | ~ 132kDa |
| NCBI Full Name: | [F-actin]-monooxygenase MICAL1 |
| UniProt Protein Name: | Protein-methionine sulfoxide oxidase MICAL1 |
| UniProt Synonym Protein Names: | Molecule interacting with CasL protein 1; MICAL-1; NEDD9-interacting protein with calponin homology and LIM domains |
| Protein Family: | Protein-methionine sulfoxide oxidase |
| UniProt Gene Name: | MICAL1 |
| UniProt Entry Name: | MICA1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MICAL1 antibody (CAB15505) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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