Name: Anti-MGST2 Antibody (CAB16400)
Brand: Metabolism Antibodies 2
SKU: CAB16400
Availability: OutOfStock

Antibody Name:	Anti-MGST2 Antibody
Antibody SKU:	CAB16400
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 50 to the C-terminus of human MGST2 (NP_002404.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Rat
Positive Samples:	U-937, PC-12

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 50 to the C-terminus of human MGST2 (NP_002404.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FRAQ QNCV EFYP IFII TLWM AGWY FNQV FATC LGLV YIYG RHLY FWGY SEAA KKRI TGFR LSLG ILAL LTLL GALG IANS FLDE YLDL NIAK KLRR QF
Gene ID:	4258
Uniprot:	Q99735
Cellular Location:	Endoplasmic reticulum membrane, Microsome membrane, Multi-pass membrane protein
Calculated MW:	8kDa/16kDa
Observed MW:	17kDa

Synonyms:

MGST2, GST2, MGST-II

Background:

The MAPEG (Membrane Associated Proteins in Eicosanoid and Glutathione metabolism) family consists of six human proteins, several of which are involved in the production of leukotrienes and prostaglandin E, important mediators of inflammation. This gene encodes a protein which catalyzes the conjugation of leukotriene A4 and reduced glutathione to produce leukotriene C4. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.

UniProt Protein Function:	MGST2: Can catalyze the production of LTC4 from LTA4 and reduced glutathione. Can catalyze the conjugation of 1-chloro-2,4- dinitrobenzene with reduced glutathione. Belongs to the MAPEG family.
UniProt Protein Details:	Protein type:Membrane protein, multi-pass; Transferase; Membrane protein, integral; Other Amino Acids Metabolism - glutathione; Endoplasmic reticulum; EC 2.5.1.18; Xenobiotic Metabolism - metabolism by cytochrome P450; Xenobiotic Metabolism - drug metabolism - cytochrome P450 Chromosomal Location of Human Ortholog: 4q28.3 Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; intracellular membrane-bound organelle; nuclear envelope; plasma membrane Molecular Function:glutathione peroxidase activity; glutathione transferase activity; leukotriene-C4 synthase activity Biological Process: glutathione biosynthetic process; leukotriene biosynthetic process; xenobiotic metabolic process
NCBI Summary:	The MAPEG (Membrane Associated Proteins in Eicosanoid and Glutathione metabolism) family consists of six human proteins, several of which are involved in the production of leukotrienes and prostaglandin E, important mediators of inflammation. This gene encodes a protein which catalyzes the conjugation of leukotriene A4 and reduced glutathione to produce leukotriene C4. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Feb 2011]
UniProt Code:	Q99735
NCBI GenInfo Identifier:	2842764
NCBI Gene ID:	4258
NCBI Accession:	Q99735.1
UniProt Secondary Accession:	Q99735,Q7Z5B8, D6RBB5,
UniProt Related Accession:	Q99735
Molecular Weight:	8,359 Da
NCBI Full Name:	Microsomal glutathione S-transferase 2
NCBI Synonym Full Names:	microsomal glutathione S-transferase 2
NCBI Official Symbol:	MGST2
NCBI Official Synonym Symbols:	GST2; MGST-II
NCBI Protein Information:	microsomal glutathione S-transferase 2
UniProt Protein Name:	Microsomal glutathione S-transferase 2
UniProt Synonym Protein Names:	Microsomal GST-II
Protein Family:	Microsomal glutathione S-transferase
UniProt Gene Name:	MGST2
UniProt Entry Name:	MGST2_HUMAN

Western blot analysis of extracts of various cell lines, using MGST2 antibody (CAB16400) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

Antibodies

MGST2 Antibody (PACO02905)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)