Cell Biology Antibodies 11

Anti-MGAT3 Antibody (CAB8134)

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SKU:
CAB8134
Product Type:
Antibody
Applications:
WB
Applications:
IHC
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:MGAT3 Rabbit Polyclonal Antibody
Antibody SKU:CAB8134
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 364-533 of human MGAT3 (NP_002400.3).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:SW620, U-251MG, SKOV3
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 364-533 of human MGAT3 (NP_002400.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:GCTV DMLQ AVYG LDGI RLRR RQYY TMPN FRQY ENRT GHIL VQWS LGSP LHFA GWHC SWCF TPEG IYFK LVSA QNGD FPRW GDYE DKRD LNYI RGLI RTGG WFDG TQQE YPPA DPSE HMYA PKYL LKNY DRFH YLLD NPYQ EPRS TAAG GWRH RGPE GRPP ARGK LDEA EV
Gene ID:4248
Uniprot:Q09327
Cellular Location:Golgi apparatus membrane, Single-pass type II membrane protein
Calculated MW:61kDa
Observed MW:61kDa
Synonyms:MGAT3, GNT-III, GNT3, beta-1
Background:There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. The enzyme encoded by this gene transfers a GlcNAc residue to the beta-linked mannose of the trimannosyl core of N-linked oligosaccharides and produces a bisecting GlcNAc. Multiple alternatively spliced variants, encoding the same protein, have been identified.
UniProt Protein Function:MGAT3: It is involved in the regulation of the biosynthesis and biological function of glycoprotein oligosaccharides. Catalyzes the addition of N-acetylglucosamine in beta 1-4 linkage to the beta-linked mannose of the trimannosyl core of N-linked sugar chains. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides. Belongs to the glycosyltransferase 17 family.
UniProt Protein Details:

Protein type:Glycan Metabolism - N-glycan biosynthesis; Transferase; EC 2.4.1.144; Membrane protein, integral

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: Golgi membrane; integral to membrane

Molecular Function:beta-1,4-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity

Biological Process: cellular protein metabolic process; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification

NCBI Summary:There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. The enzyme encoded by this gene transfers a GlcNAc residue to the beta-linked mannose of the trimannosyl core of N-linked oligosaccharides and produces a bisecting GlcNAc. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:Q09327
NCBI GenInfo Identifier:61252497
NCBI Gene ID:4248
NCBI Accession:Q09327.3
UniProt Secondary Accession:Q09327,Q14CK5, Q6IC49, Q9UH32, A6NGD0,
UniProt Related Accession:Q09327
Molecular Weight:61,313 Da
NCBI Full Name:Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase
NCBI Synonym Full Names:mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase
NCBI Official Symbol:MGAT3  
NCBI Official Synonym Symbols:GNT3; GNT-III  
NCBI Protein Information:beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase; GlcNAc-T III; N-acetylglucosaminyltransferase III; N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase III
UniProt Protein Name:Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase
UniProt Synonym Protein Names:N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase III; GNT-III; GlcNAc-T III; N-acetylglucosaminyltransferase III
Protein Family:Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase
UniProt Gene Name:MGAT3  
UniProt Entry Name:MGAT3_HUMAN
Anti-MGAT3 Polyclonal Antibody (CAB8134)Western blot analysis of extracts of various cell lines, using MGAT3 antibody (CAB8134) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
Anti-MGAT3 Polyclonal Antibody (CAB8134)Immunohistochemistry of paraffin-embedded rat heart using MGAT3 antibody (CAB8134) at dilution of 1:100 (40x lens).
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