Cell Biology Antibodies 12

Anti-MGAT2 Antibody (CAB9134)

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SKU:
CAB9134
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-MGAT2 Antibody
Antibody SKU:CAB9134
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 178-447 of human MGAT2 (NP_002399.1).
Application:WB IHC
Recommended Dilution:WB 1:200 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:K-562, Mouse kidney, Rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 178-447 of human MGAT2 (NP_002399.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:PFSI QLYP NEFP GSDP RDCP RDLP KNAA LKLG CINA EYPD SFGH YREA KFSQ TKHH WWWK LHFV WERV KILR DYAG LILF LEED HYLA PDFY HVFK KMWK LKQQ ECPE CDVL SLGT YSAS RSFY GMAD KVDV KTWK STEH NMGL ALTR NAYQ KLIE CTDT FCTY DDYN WDWT LQYL TVSC LPKF WKVL VPQI PRIF HAGD CGMH HKKT CRPS TQSA QIES LLNN NKQY MFPE TLTI SEKF TVVA ISPP RKNG GWGD IRDH ELCK SYRR LQ
Gene ID:4247
Uniprot:Q10469
Cellular Location:Golgi apparatus membrane, Single-pass type II membrane protein
Calculated MW:51kDa
Observed MW:60kDa
Synonyms:MGAT2, CDG2A, CDGS2, GLCNACTII, GNT-II, GNT2, alpha-1
Background:The product of this gene is a Golgi enzyme catalyzing an essential step in the conversion of oligomannose to complex N-glycans. The enzyme has the typical glycosyltransferase domains: a short N-terminal cytoplasmic domain, a hydrophobic non-cleavable signal-anchor domain, and a C-terminal catalytic domain. Mutations in this gene may lead to carbohydrate-deficient glycoprotein syndrome, type II. The coding region of this gene is intronless. Transcript variants with a spliced 5' UTR may exist, but their biological validity has not been determined.
UniProt Protein Function:Catalyzes an essential step in the conversion of oligo-mannose to complex N-glycans.
NCBI Summary:The product of this gene is a Golgi enzyme catalyzing an essential step in the conversion of oligomannose to complex N-glycans. The enzyme has the typical glycosyltransferase domains: a short N-terminal cytoplasmic domain, a hydrophobic non-cleavable signal-anchor domain, and a C-terminal catalytic domain. Mutations in this gene may lead to carbohydrate-deficient glycoprotein syndrome, type II. The coding region of this gene is intronless. Transcript variants with a spliced 5' UTR may exist, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
UniProt Code:Q10469
NCBI GenInfo Identifier:1708004
NCBI Gene ID:4247
NCBI Accession:Q10469.1
UniProt Related Accession:Q10469
Molecular Weight:~ 60kDa
NCBI Full Name:Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
NCBI Synonym Full Names:alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
NCBI Official Symbol:MGAT2  
NCBI Official Synonym Symbols:GNT2; CDG2A; CDGS2; GNT-II; GLCNACTII  
NCBI Protein Information:alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
UniProt Protein Name:Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
UniProt Synonym Protein Names:Beta-1,2-N-acetylglucosaminyltransferase II; GlcNAc-T II; GNT-II; Mannoside acetylglucosaminyltransferase 2; N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II
Protein Family:Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
UniProt Gene Name:MGAT2  
Anti-MGAT2 Polyclonal Antibody (CAB9134)Western blot analysis of extracts of various cell lines, using MGAT2 antibody (CAB9134) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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