Name: Anti-MGAT1 Antibody (CAB12459)
Brand: Cell Biology Antibodies 3
SKU: CAB12459
Availability: OutOfStock

Antibody Name:	Anti-MGAT1 Antibody
Antibody SKU:	CAB12459
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 156-445 of human MGAT1 (NP_001108092.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	SKOV3, MCF7

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 156-445 of human MGAT1 (NP_001108092.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GSAV THIR QPDL SSIA VPPD HRKF QGYY KIAR HYRW ALGQ VFRQ FRFP AAVV VEDD LEVA PDFF EYFR ATYP LLKA DPSL WCVS AWND NGKE QMVD ASRP ELLY RTDF FPGL GWLL LAEL WAEL EPKW PKAF WDDW MRRP EQRQ GRAC IRPE ISRT MTFG RKGV SHGQ FFDQ HLKF IKLN QQFV HFTQ LDLS YLQR EAYD RDFL ARVY GAPQ LQVE KVRT NDRK ELGE VRVQ YTGR DSFK AFAK ALGV MDDL KSGV PRAG YRGI VTFQ FRGR RVHL APPL TWEG YDPS WN
Gene ID:	4245
Uniprot:	P26572
Cellular Location:	Golgi apparatus membrane, Single-pass type II membrane protein
Calculated MW:	50kDa
Observed MW:	60kDa

Synonyms:

MGAT1, GLCNAC-TI, GLCT1, GLYT1, GNT-1, GNT-I, GnTI, MGAT, alpha-1

Background:

There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1, 2-N-acetylglucosaminyltransferase I is a medial-Golgi enzyme essential for the synthesis of hybrid and complex N-glycans. The protein, encoded by a single exon, shows typical features of a type II transmembrane protein. The protein is believed to be essential for normal embryogenesis. Several variants encoding the same protein have been found for this gene.

UniProt Protein Function:	MGAT1: Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans. Belongs to the glycosyltransferase 13 family.
UniProt Protein Details:	Protein type:EC 2.4.1.101; Glycan Metabolism - N-glycan biosynthesis; Membrane protein, integral; Transferase Chromosomal Location of Human Ortholog: 5q35.3 Cellular Component: Golgi membrane; membrane Molecular Function:acetylglucosaminyltransferase activity; alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity Biological Process: carbohydrate metabolic process
NCBI Summary:	There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I is a medial-Golgi enzyme essential for the synthesis of hybrid and complex N-glycans. The protein, encoded by a single exon, shows typical features of a type II transmembrane protein. The protein is believed to be essential for normal embryogenesis. Several variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	P26572
NCBI GenInfo Identifier:	311033399
NCBI Gene ID:	4245
NCBI Accession:	P26572.2
UniProt Secondary Accession:	P26572,Q6IBE3, A8K404, B3KRU8, D3DWR1,
UniProt Related Accession:	P26572
Molecular Weight:	51kDa
NCBI Full Name:	Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
NCBI Synonym Full Names:	mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase
NCBI Official Symbol:	MGAT1
NCBI Official Synonym Symbols:	GnTI; MGAT; GLCT1; GLYT1; GNT-1; GNT-I; GLCNAC-TI
NCBI Protein Information:	alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
UniProt Protein Name:	Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
UniProt Synonym Protein Names:	N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I; GNT-I; GlcNAc-T I
UniProt Gene Name:	MGAT1

Western blot analysis of extracts of various cell lines, using MGAT1 antibody (CAB12459) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

Anti-MGAT1 Antibody (CAB6387)

MGAT1 Antibody (PACO10476)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)